Diagnosis of EB virus IgM,IgG and DNA in children with respiratory infectious diseases
Objective To explore the diagnostic value of Epstein-Barr virus(EBV)immunoglobulin(Ig)M,IgG and DNA in children with respiratory infectious diseases.Methods 400 children with respiratory infectious diseases were selected as the observation group and 100 children without respiratory tract infection as the control group.EBV-IgM,EBV-IgG and EBV-DNA were detected in the two groups.The positive rates of EBV-IgM,EBV-IgG and EBV-DNA in children of different ages in the observation group were compared,as well as the detection results of EBV-IgM,EBV-IgG and EBV-DNA between the two groups.The diagnostic value of each index was analyzed.Results The positive rates of EBV-IgM and EBV-IgG in children of different age groups in the observation group were compared,and the difference was statistically significant(P<0.05).There was no significant difference in the positive rate of EBV-DNA in children of different age groups in the observation group(P>0.05).In EBV-IgM detection,the chemiluminescence value of(11.39±2.12)in the observation group was higher than(9.54±1.35)in the control group,and the positive rate of 50.50%(202/400)was higher than 5.00%(5/100)in the control group.The difference was statistically significant(P<0.05).In EBV-IgG detection,the absorbance value of(11.33±2.15)in the observation group was higher than(9.03±1.06)in the control group,and the positive rate of 58.00%(232/400)was higher than 12.00%(12/100)in the control group.The difference was statistically significant(P<0.05).In EBV-DNA detection,the Ct value of cycle number of(34.13±4.88)in the observation group was lower than(43.75±11.62)in the control group,and the positive rate of 57.00%(228/400)was higher than 6.00%(6/100)in the control group.The difference was statistically significant(P<0.05).EBV-DNA has the highest diagnostic value for children with respiratory infectious diseases[area under the curve(AUC)=0.644],followed by EBV-IgM(AUC=0.618),EBV-IgG(AUC=0.584),and EBV-IgM has the highest diagnostic specificity for children with respiratory infectious diseases(96.12%).Conclusion The detection of EBV-IgM,EBV-IgG and EBV-DNA has important guiding significance in the diagnosis of respiratory infectious diseases in children.
Epstein-Barr virusImmunoglobulin MImmunoglobulin GDeoxyribonucleic acidRespiratory infectious diseases in children
万方数据
维普
