Repair of 2%supramolecular salicylic acid on UVB induced photoaging in mice
Objective To explore the repair of 2%supramolecular salicylic acid on ultraviolet B(UVB)induced photoaging in mice.Methods 30 Kunming female rats aged 6-8 weeks were selected and randomly divided into a blank control group,a UVB group,and a UVB+2%supramolecular salicylic acid group,each with 10 rats.The blank control group was fed under normal sunlight irradiation,the UVB group and the UVB+2%supramolecular salicylic acid group were irradiated with UVB once every other day for 12 weeks,in which the UVB+2%supramolecular salicylic acid group was applied with 2%supramolecular salicylic acid once after irradiation and once daily on non irradiation days.48 h after the end of the last irradiation,the morphological changes of the skin tissue of each group of mice were observed,hematoxylin-eosin(HE)staining was used to observe the structural changes of the dorsal skin,and measure the thickness of the epidermis and dermis.Masson staining was used to observe the arrangement of collagen fibers in the dermis,and measure the content of collagen fibers;immunohistochemical staining was used to observe the expression of p21 and p53 in the skin tissue.The dorsal skin appearance,skin microstructural changes and positive expression of p21 and p53 in skin tissues of three groups of mice were compared.Results Compared with the blank control group,the skin of mice in the UVB group was rough,with deep wrinkles,enlarged pores,and lack of elasticity;the skin presents brown yellow spots,with a leather like appearance.Microscopically,capillary dilation and crusting are seen,which were typical characteristics of photoaging.However,the skin of mice in the UVB+2%supramolecular salicylic acid group had faint brown spots,and under microscope,there were scattered and irregular brown patches on the skin,with less photoaging symptoms.There was significant difference in the degree of dorsal skin injury among the three groups(P<0.05).The degree of dorsal skin injury in UVB group was obviously greater than that of blank control group and UVB+2%supramolecular salicylic acid group,and there was a significant difference(P<0.05).There was also significant difference in degree of skin lesion between the blank control group and UVB+2%supramolecular salicylic acid group(P<0.05).The epidermal thickness,dermal thickness and dermal collagen fiber content of the blank control group were(18.55±1.80)μm,(350.08±35.16)μm and(0.46±0.05)OD,those of the UVB group were(79.68±21.37)μm,(611.62±16.37)μm and(0.15±0.30)OD,and those of UVB+2%supramolecular salicylic acid group were(60.99±11.49)μm,(467.94±20.71)μm and(0.32±0.03)OD.There was significant difference in epidermal thickness,dermal thickness and dermal collagen fiber content among the three groups(P<0.05).The epidermal thickness and dermal thickness in the VB+2%supramolecular salicylic acid group and UVB group were obviously more than those in the blank control group;the dermal collagen fiber content was significant less than that in the blank control group,and there was a significant difference(P<0.05).The epidermal thickness and dermal thickness in the VB+2%supramolecular salicylic acid group were obviously less than that in UVB group,and the dermal collagen fiber content was more than that in UVB group,and there was a significant difference(P<0.05).Immunohistochemistry showed no positive expression of p21 and p53 in the blank control group.The positive expression rates of p21 and p53 in UVB+2%supramolecular salicylic acid group were 30.00%and 10.00%,which were lower than 80.00%and 60.00%in UVB group,and there was a significant difference(P<0.05).Conclusion 2%supramolecular salicylic acid has a repairing and protective effect on UVB induced photoaging skin in mice,and its mechanism is related to inhibiting the expression of apoptosis related genes p21 and p53.
Skin photoaging2%supramolecular salicylic acidUltraviolet B
NETL
NSTL
万方数据
维普
