The mechanism of Luohua Zizhu granules in the treatment of pharyngitis based on HPLC-Q-Exactive Orbitrap-MS and network pharmacology
Objective To preliminarily explore the active ingredients and potential mechanism of Luohua Zizhu granules in the treatment of pharyngitis based on high performance liquid chromatography-Q-Exactive Orbitrap-mass spectrometry(HPLC-Q-Exactive Orbitrap-MS)and network pharmacology.Methods HPLC-Q-Exactive Orbitrap-MS was used to analyze and identify the main chemical constituents of Luohua Zizhu granules,and the qualitative compounds were recorded into TCMSP,HERB and Swiss Target databases to screen the component targets.The search term"pharyngitis"was used to search for the corresponding target in GeneCards,DisGeNET,Drugbank and other databases.The intersection of the chemical constituents of Luohua Zizhu granules and the disease targets was plotted as a Wayne diagram,and then the Chinese medicine-compound-target network was constructed and visualized by using Cytoscape 3.9.1 software,and the GO functional enrichment and KEGG pathway enrichment analyses were carried out through the Metascape database and the bubble diagrams were plotted for visualization.Results A total of 18 main chemical components were identified from the granules.The database screened 155 corresponding targets of chemical components,2319 corresponding targets of pharyngitis,and 76 corresponding targets were obtained by taking the intersection,and 38 key targets were judged according to the network topology parameters.The key targets involved serine/threonine protein kinase 1(Akt1),tumor necrosis factor(TNF),tumor suppressor protein P53(TP53),interleukin-6(IL-6)and vascular endothelial growth factor A(VEGFA).GO functional enrichment analysis yielded 269 entries(P<0.01),and KEGG pathway enrichment analysis screened 116 signaling pathways.Conclusion The compounds in Luohua Zizhu granules can prevent and control pharyngitis through multi-components,multi-targets,and multi-pathways,and their molecular mechanisms are related to the targets of Akt1,TNF,TP53,IL-6,and VEGFA,and the components of apigenin,lignocerol,and caffeic acid,which are involved in the TNF pathway,the interleukin-17(IL-17)pathway,the phosphatidylinositol 3-kinase(PI3K)-Akt pathway,the nuclear factor-κB(NF-κB)pathway,the toll-like receptor pathway and the mitogen-activated protein kinase(MAPK)pathway,the cancer pathway and the polysaccharide proteins and apoptosis.It lays a theoretical foundation for subsequent multi-target drug development,molecular biology experiments and related clinical studies.
High performance liquid chromatography-Q-Exactive Orbitrap-mass spectrometryNetwork pharmacologyLuohua Zizhu granulesPharyngitisMechanism
万方数据
维普
