Comparison of the value of enzyme-linked immunosorbent assay and Western blot for the detection of anti-nucleosome antibodies in the diagnosis of SLE
Objective To compare the value of enzyme-linked immunosorbent assay(ELISA)and Western blot(WB)for the detection of anti-nucleosome antibodies(AnuA)in the diagnosis of systemic lupus erythematosus(SLE).Methods 306 patients with autoimmune diseases were divided into SLE group(SLE patients,144 cases)and non-SLE group(non-SLE patients,162 cases)according to clear clinical diagnosis.100 patients with non-autoimmune diseases in the same period were selected as the control group.All study subjects were tested for AnuA by ELISA and WB.The AnuA detection results of the two detection methods were counted separately.The diagnostic value of the two detection methods for AnuA was compared,including accuracy,sensitivity,specificity,positive predictive value,negative predictive value,and statistical tests were performed to analyze the consistency of the two detection methods.Results In SLE group:120 cases were positive and 24 cases were negative for AnuA by ELISA,109 cases were positive and 35 cases were negative for AnuA by WB;in non-SLE group:76 cases were positive and 86 cases were negative for AnuA by ELISA,71 cases were positive and 91 cases were negative for AnuA by WB;in control group:17 cases were positive and 83 cases were negative for AnuA by ELISA,11 cases were positive and 89 cases were negative for AnuA by WB.There was no significant difference in AnuA positive rate among SLE group,non-SLE group and control group(P>0.05).However,the AnuA positive rate of patients in the SLE group was significantly higher than that of the non-SLE group and the control group for both methods(P<0.05).The sensitivity of ELISA for AnuA was 87.4%(180/206),which was higher than 80.1%(165/206)of WB(P<0.05).The specificity,positive predictive value,negative predictive value and accuracy of the two detection methods were not statistically significant(P>0.05).The total coincidence rate of the two detection methods was 90.1%,and the judgment has a good consistency(K value=0.516).Conclusion ELISA and WB detection of AnuA have high diagnostic efficiency in SLE,and WB can be used in the initial detection of AnuA in clinical practice.If necessary,ELISA can be used in retesting to effectively improve the positive rate of the test and provide reference for clinical diagnosis of SLE.
Anti-nucleosome antibodiesSystemic lupus erythematosusWestern blot assayEnzyme-linked immunosorbent assay
万方数据
维普
