Study on the involvement of circRNA_0002120 in the mechanism of angiogenesis after acute ischemic stroke by regulating the expression of VEGF and EGF
Objective To evaluate the expression level of circular RNAs(circRNAs)in the blood of patients with acute ischemic stroke,and confirm that circRNAs can be used as a novel biomarker for acute ischemic stroke.Methods CircRNAs closely related to angiogenesis after acute ischemic stroke were mined from the known Genome data,and the potential target genes or proteins mediated by circRNAs in angiogenesis after acute ischemic stroke were predicted and analyzed by KEGG/GO.Circular base(circBase),and circular network database(circNet database)confirmed the expression of circRNAs in brain tissues and in peripheral blood.The clinical data of 30 patients with acute ischemic stroke(acute ischemic stroke group)and 30 healthy subjects(control group)were collected.The expression levels of circRNA,vascular endothelial growth factor(VEGF)and epidermal growth factor(EGF)were compared between the two groups.Results CircRNA_0002120 was predicted by bioinformatics to be closely related to angiogenesis after acute ischemic stroke,and its target genes include VEGF and EGF.It was confirmed by real-time quantitative polymerase chain reaction(qRT-PCR)that the expression of Hsa circRNA_0002120(PAPOLA)was increased in the acute ischemic stroke group[(1.13±0.02)]when compared with the control group[(0.99±0.01)],and the difference was statistically significant(t=34.293,P=0.000<0.01).Enzyme-linked immunosorbent assay(ELISA)confirmed that serum VEGF concentration in the acute ischemic stroke group[(317.80±10.53)pg/ml]was significantly increased when compared with the control group[(168.80±4.89)pg/ml],and the difference was statistically significant(t=70.293,P=0.000<0.0001).The serum VEGF concentration in the acute ischemic stroke group[(150.70±5.17)pg/ml]was significantly increased when compared with the control group[(86.38±3.84)pg/ml],and the difference was statistically significant(t=54.704,P=0.000<0.0001).Conclusion Hsa circRNA_0002120 can be used as a biomarker of acute ischemic stroke,regulate the angiogenesis after cerebral infarction,and promote the recovery of nerve function.
万方数据
维普
