Objective To detect the expression of Tn antigen in patients with endometrial carcinoma and to investi-gate its biological significance.Methods We first used immunohistochemical staining to detect the expression and local-ization of Tn antigen in human endometrial carcinoma tissues,atypical endometrial tissues,and normal endometrial tis-sues.We then used the CRISPR/Cas9 editing system to deplete the Cosmc gene in two human endometrial carcinoma cell lines(Ishikawa,HEC-1B)to forcedly induce Tn antigen expression.The Tn antigen expression was determined by flow cytometric analysis.Trans well assays were performed to measure cell migration and invasion.Finally,we used western blot analysis to assess the effects of Tn expression on induction of the epithelial-mesenchymal transition(EMT)signaling pathway in cells.Results It showed that Tn antigen was not detected in normal endometrial tissues but lowly expressed in atypical endometrial hyperplasia and highly expressed in endometrial carcinoma tissues.Depletion of Cosmc forcedly induced the expression of Tn antigen in two cancer cell lines and subsequently promoted cell migration and in-vasion capabilities.In addition,the EMT program was significantly activated following Tn antigen expression,demon-strated by a down-regulation of E-cadherin and an up-regulation of Vimentin and N-cadherin.Conclusion Tn antigen is highly expressed in human endometrial carcinoma and may contribute to cancer cell migration and invasion through acti-vation of the EMT signaling pathway.
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