摘要
目的 探究miR-101-3p与膀胱癌细胞增殖、迁移、侵袭和顺铂敏感性的关系.方法 将膀胱癌细胞分为对照组、下、上调miR-101-3p组,体外培养膀胱癌细胞.采用CCK-8实验来检测膀胱癌细胞增殖能力;通过划痕实验来检测膀胱癌细胞迁移能力;通过Transwell实验来检测膀胱癌细胞侵袭能力;3组细胞分别加入不同浓度的顺铂,观察对细胞的抑制率;Western blot法测定CDK4、Akt、ICAM-1、MMP-9蛋白的表达量.结果 在24 h、48 h、72 h的细胞增殖率方面,上调miR-101-3p组均低于对照组、下调miR-101-3p组,而对照组低于下调miR-101-3p组(P＜0.05);在24 h、48 h、72 h的细胞迁移数、侵袭数方面,上调miR-101-3p组均少于对照组、下调miR-101-3p组,而对照组少于下调miR-101-3p组(P＜0.05);在CDK4、Akt、ICAM-1、MMP-9蛋白的表达量方面,上调miR-101-3p组均低于对照组和下调miR-101-3p组(P＜0.05);在顺铂敏感度方面,上调miR-101-3p组高于对照组、下调miR-101-3p组(P＜0.05).结论 上调miR-101-3p能够抑制CDK4、Akt、ICAM-1、MMP-9蛋白的表达,调控细胞周期,阻止膀胱癌细胞的增殖、迁移、侵袭,提高其顺铂敏感性.
Abstract
Objective To explore the relationship between miR-101-3p and bladder cancer cell proliferation,migra-tion,invasion and cisplatin sensitivity.Methods Bladder cancer cells were divided into control group,down-and up-reg-ulated miR-101-3p groups,and bladder cancer cells were cultured in vitro.The proliferation ability of bladder cancer cells was detected by CCK-8 assay;the migration ability of bladder cancer cells was detected by scratch assay;the inva-sion ability of bladder cancer cells was detected by Transwell assay;three groups of cells were added with different con-centrations of cisplatin,and the cells The inhibition rate of CDK4,Akt,ICAM-1 and MMP-9 protein was determined by Western blot.Results In terms of the cell proliferation rate at 24 h,48 h,and 72 h,The upregulated miR-101-3p group were all lower than the control group,and the downregulated miR-101-3p group,However,the control group was lower than the miR-101-3p knockdown group(P＜0.05);In terms of the number of cell migration and invasion at 24 h,48 h,and 72 h,The upregulated miR-101-3p group were less than the control group,and the downregulated miR-101-3p group,However,the control group was less downregulated than the miR-101-3p knockdown group(P＜0.05);In terms of the expression levels of the CDK4,Akt,ICAM-1,and MMP-9 proteins,The miR-101-3p group was lower than the control group and the miR-101-3p group(P＜0.05);In terms of the cisplatin sensitivity,The miR-101-3p group was higher than the control group and the miR-101-3p group(P＜0.05).Conclusion Up-regulation of miR-101-3p can in-hibit the expression of CDK4,Akt,ICAM-1 and MMP-9 proteins,regulate the cell cycle,block the proliferation,migra-tion and invasion of bladder cancer cells,and improve their cisplatin sensitivity.
基金项目
佳木斯大学博士专项科研基金启动项目(JMSUBZ2021-14)
黑龙江省省属本科高等学校基本科研业务费科技项目(2022-KYYWF-0634)
NETL
NSTL
维普
万方数据