Objective To explore the relationship between miR-101-3p and bladder cancer cell proliferation,migra-tion,invasion and cisplatin sensitivity.Methods Bladder cancer cells were divided into control group,down-and up-reg-ulated miR-101-3p groups,and bladder cancer cells were cultured in vitro.The proliferation ability of bladder cancer cells was detected by CCK-8 assay;the migration ability of bladder cancer cells was detected by scratch assay;the inva-sion ability of bladder cancer cells was detected by Transwell assay;three groups of cells were added with different con-centrations of cisplatin,and the cells The inhibition rate of CDK4,Akt,ICAM-1 and MMP-9 protein was determined by Western blot.Results In terms of the cell proliferation rate at 24 h,48 h,and 72 h,The upregulated miR-101-3p group were all lower than the control group,and the downregulated miR-101-3p group,However,the control group was lower than the miR-101-3p knockdown group(P＜0.05);In terms of the number of cell migration and invasion at 24 h,48 h,and 72 h,The upregulated miR-101-3p group were less than the control group,and the downregulated miR-101-3p group,However,the control group was less downregulated than the miR-101-3p knockdown group(P＜0.05);In terms of the expression levels of the CDK4,Akt,ICAM-1,and MMP-9 proteins,The miR-101-3p group was lower than the control group and the miR-101-3p group(P＜0.05);In terms of the cisplatin sensitivity,The miR-101-3p group was higher than the control group and the miR-101-3p group(P＜0.05).Conclusion Up-regulation of miR-101-3p can in-hibit the expression of CDK4,Akt,ICAM-1 and MMP-9 proteins,regulate the cell cycle,block the proliferation,migra-tion and invasion of bladder cancer cells,and improve their cisplatin sensitivity.
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NSTL
维普
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