间充质干细胞膜包载5-氨基酮戊酸介导的光热作用抑制皮脂分泌
Research on the inhibition of sebum secretion in acne treatment by mesenchymal stem cell membrane-encapsulated 5-amin-olevulinic acid-mediated photothermal therapy
赵天奇 1于然 1王余海 1朱明姬1
作者信息
- 1. 吉林大学中日联谊医院皮肤科,吉林长春 130033
- 折叠
摘要
目的 探究间充质干细胞膜包载5-氨基酮戊酸(ALA@STCM)介导的光热作用对金黄地鼠皮脂腺斑皮脂分泌的影响.方法 培养脐带来源的间充质干细胞(MSCs),提取MSC膜,制备ALA@STCM.首先,通过CCK-8实验对比5-氨基酮戊酸(ALA)和ALA@STCM对皮脂腺细胞活力的影响.其次,建立金黄地鼠痤疮动物模型,通过组织学分析对比ALA和ALA@STCM对皮脂分泌的影响.结果 CCK-8结果表明,相较于ALA,使用MSC膜包载ALA未明显增加细胞毒性,证明了 MSC膜作为载药工具的安全性.组织学分析结果表明,给予光照后,ALA和ALA@STCM均能不同程度地缩小皮脂腺斑面积和抑制皮脂分泌,但是我们发现ALA@STCM的缩小皮脂腺斑的作用和抑制皮脂分泌作用明显优于ALA.结论 ALA@STCM介导的光热作用可缩小金黄地鼠的皮脂腺斑、抑制皮脂分泌,并且显著优于ALA.
Abstract
Objective This study aimed to explore the impact of photothermal therapy mediated by mesenchymal stem cell membrane-coated 5-aminolevulinic acid(ALA@STCM)on sebum secretion in sebaceous gland hyperplasia.Methods Mesenchymal stem cells(MSCs)derived from umbilical cords were cultured,and MSC membranes were ex-tracted to prepare ALA@STCM.Firstly,the effect of 5-aminolevulinic acid(ALA)and ALA@STCM on the viability of sebaceous gland cells was compared using a CCK-8 assay.Secondly,a golden hamster acne animal model was estab-lished,and histological analysis was conducted to compare the effects of ALA and ALA@STCM on sebum secretion.Results The CCK-8 assay results indicated that,compared to ALA,the use of MSC membrane-coated ALA did not significantly increase cytotoxicity,confirming the safety of MSC membrane as a drug delivery tool.Histological analysis results revealed that,follo wing light exposure,both ALA and ALA@STCM could reduce sebaceous gland hyperplasia and inhibit sebum secretion to varying degrees.However,it was observed that AL A@STCM had a significantly greater effect in reducing sebaceous gland hyperplasia and inhibiting sebum secretion compared to ALA alone.Conclusion Pho-tothermal effects mediated by ALA@STCM can effectively reduce sebaceous gland hyperplasia in golden hamsters,in-hibit sebum secretion,and exhibit a significant advantage over ALA in achieving these effects.
关键词
寻常痤疮/5-氨基酮戊酸/间充质干细胞/光热作用Key words
Acne Vulgaris/5-Aminolevulinic Acid/Mesenchymal Stem Cells/Photothermal Effects引用本文复制引用
基金项目
吉林省科技发展计划项目(20220204004YY)
出版年
2024
NETL
NSTL
万方数据