Objective To investigate the effects of miR-126,miR-221,miR-155 and peripheral blood T cell expres-sion on plaque stability in patients with coronary heart disease.Methods 138 patients with coronary heart disease trea-ted in the Department of Cardiology of our hospital from February 2019 to June 2020 were selected.According to the nature of plaque evaluated by intravascular ultrasound,they were divided into stable plaque group(56 cases)and unsta-ble plaque group(82 cases).Another 60 healthy volunteers were collected as the control group.The relative expressions of miR-126,miR-221 and miR-155 in plasma were detected by real-time fluorescence quantitative PCR;The proportion of CD4+CD25+Foxp3+regulatory T cells in CD4+cells was determined by flow cytometry;Four items of blood lipid were detected by automatic biochemical analyzer,and serum was detected by enzyme-linked immunosorbent assay y In-terferon(IFN-γ)Level.Results Compared with the control group,the expression levels of miR-126 were significantly reduced in the stable and unstable plaque groups,while the expression levels of miR-221 and miR-155 were significantly increased,with a statistically significant difference(P＜0.05);The expression levels of miR-126 in unstable plaque group were significantly lower than those in stable plaque group,and the expression levels of miR-221 and miR-155 were significantly higher than those in stable plaque group(P＜0.05).Compared with the control group,the proportion of CD4+CD25+Foxp3+regulatory T cells in CD4+cells was significantly reduced in patients with stable and unstable plaques,and the level of serum IFN-γ has significantly increased(P＜0.05);The proportion of CD4+CD25+Foxp3+regulatory T cells in CD4+cells in unstable plaque group was significantly lower than that in stable plaque group,and the serum IFN-γ level was significantly higher than that in the stable plaque group(P＜0.05).Compared with the con-trol group,the levels of total cholesterol,triglycerides,and low-density lipoprotein cholesterol in patients with stable and unstable plaques were significantly increased,the levels of high-density lipoportein cholesterol were significantly re-duced,with a statistically significant difference(P＜0.05).The expression level of miR-126 in patients with coronary heart disease was negatively correlated with the level of total cholesterol and low-density lipoprotein cholesterol,and positively correlated with the proportion of CD4 1 CD25+Foxp3+regulatory T cells in CD4+cells.The expression levels of miR-221 and miR-155 in patients with coronary heart disease were positively correlated with the levels of total cho-lesterol,triglyceride and low-density lipoprotein cholesterol,and negatively correlated with the proportion of CD4 1 CD25+Foxp3+regulatory T cells in CD4+cells.Conclusion The expression levels of miR-126,miR-221 and miR-155 were closely related to coronary plaque stability,CD4+regulatory T cell expression and blood lipid levels in patients with coronary heart disease.
coronary heart diseasemiR-126miR-221miR-155regulatory T cellsatherosclerosis
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