Objective To investigate the expression of hsa_circ_MIB1 in glioma and its clinical significance.Methods High-throughput sequencing was used to detect the differentially expressed circRNAs in five glioblastoma tissues and four normal brain tissues,and hsa_circ_MIB1,which was significantly down-regulated,was selected and identified as the study target.The expression levels of hsa_circ_MIB1 in glioma tissues and normal brain tissues and glial cell line HEB and three glioblastoma cell lines(U251,U87,SF126)were detected and compared using real-time fluorescence quantita-tive PCR.The relationship between hsa_circ_MIB1 expression levels and clinicopathological characteristics was ana-lyzed;the Kaplan-meier method was used to analyze the relationship between hsa_circ_MIB1 expression levels and sur-vival;subject operating characteristic(ROC)curves were constructed to assess the effect of hsa_circ_MIB1 on glioma.The sensitivity and specificity of hsa_circ_MIB1 for glioma diagnosis were assessed by constructing receiver operating characteristic(ROC)curves.Results The expression of hsa_circ_MIB1 in glioma tissues(0.28±0.63)was significant-ly lower than that in normal brain tissues(1.60±1.56),and the difference was statistically significant(P＜0.0001).The expression levels of hsa_circ_MIB1 in all malignant glioma cell lines were significantly lower than those in glial cell lines(P＜0.05).hsa_circ_MIB1 expression was significantly correlated with the clinical stage of glioma patients(x2=7.930,P＜0.005),and the higher the clinical stage,the lower the expression level of hsa_circ_MIB1 Kaplan-Meier sur-vival quality analysis showed that glioma patients with low hsa circ MIB1 expression had significantly longer survival than those with high hsa_circ_MIB1 expression;the critical value of the ROC curve for diagnosis was 0.8289 and the area under the curve was 0.928,which corresponded to a sensitivity and specificity of 100.0％and 82.89％,respective-ly.Conclusion hsa_circ_MIB1 expression is decreased in glioma,and its low expression correlates with clinical staging and prognosis,and can be used as a novel molecular marker for glioma diagnosis and treatment.
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