Objective To evaluate the clinical application of improved and optimized method for preparing bone mar-row chromosomes combined with fluorescence in situ hybridization(FISH)technique in the diagnosis of hematological malignancies,especially in chronic myeloid leukemia(CML).Methods A total of 355 patients with hematological malig-nancies in Qingyuan People's Hospital from January 2019 to June 2022 were enrolled in this study.Firstly,marrow cells from 16 patients were employed to optimized the bone marrow chromosomes preparing method.Then,each case was equally divided into 4 groups(A,B,C and D groups)according to the different culture time(16,20,24 and 28 h)and one-way analysis of variance was performed.The improved method was then used to detect bone marrow karyotype in all patients.In addition,FISH technique was used to detect the BCR-ABL fusion gene in CML patients,and abnormal chromosomes were statistically analyzed.Results One-way analysis of variance found that there was a significant difference in the mean value of chromosome division among the four groups(P＜0.05),and group B(20 h)was signifi-cantly higher than group D(28 h,P＜0.05).The culture success rate of group B was also significantly higher than that of group D(P＜0.05).The uniform culture success rate of 355 patients with hematological malignancies was 96.9％(344/355),and the detection rate of abnormal chromosomes was 40.3％(143/355).The positive detection rates of chromosomes and FISH in the bone marrow of CML patients were consistent,and additional abnormal chromosomes were detected in the accelerated phase and blast phase.Conclusion The improved short-term culture method with 20 hours of culture has high success rate,good stability,and high detection rate of abnormal chromosomes,which is the best solution for the preparation of bone marrow chromosomes.Ph chromosomes and additional abnormal chromosomes are closely related to the course of CML.The detection of chromosome abnormalities provides an important reference for clinical diagnosis,classification,subsequent treatment and prognosis of leukemia.
bone marrow chromosomesfluorescence in situ hybridizationchromosome abnormalitieshematological malignancies
NETL
NSTL
万方数据
