Objective Analyze the drug-resistance mechanism and molecular epidemiological characteristics of clini-cal and environmental multidrug-resistant Acinetobacter baumannii(MDRAB)in hospitals,providing evidence for the prevention and control of the spread of MDRAB.Methods Collected 34 non-repetitive MDRAB strains which isolated from the clinic or environment in hospital from May 2020 to April 2021,including 25 clinical specimens and 9 environ-mental specimens.Detecting the resistance genes of MDRAB by PCR and gene Sequencing.And Multilocus sequence typing(MLST)and Pulsed field gel electrophoresis(PFGE)were used for typing the molecular type of MDRAB.Results The positive gene carrying rates of the 34 MDRAB are:blaOXA23,blaOXA-51and blaOXA64gpare all 100％,TEM 85.3％(29/34).7 ST sequence types were monitored in 25 clinical MDRAB strains,inclouding ST540(10/25),ST195(5/25),ST369(3/25),ST208(2/25),ST381(2/25),ST938(2/25),ST451(1/25),6 clone groups:Group A(9/25),Group D(8/25),Group C(4/25),Group F(2/25),Group E(1/25),Group G(1/25).2 ST sequence types were detected in 9 strains of environmental MDRAB:ST208(8/9)and ST540(1/9),2 clone groups:Group B(8/9),Group C(1/9).Conclusion MDRAB in the hospital mainly comes from the intensive care unit,the main Carbapenem resistance mode are blaOXA-51-Like+blaOXA-23+blaTEM-like.The main popular sequence types in hospital are ST540 and ST195,and the main clone groups are group A and group D.ST540 and clone Group A runs through the entire research phase and spreads in multiple intensive care units.The main ST sequence types and clonal Groups are different between clini-cal and environmental MDRAB.
multidrug-resistant acinetobacter baumannii(MDRAB)multilocus sequence typing(MLST)pulsed field gel electrophoresis(PFGE)homology
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