Objective To investigate the urinary characteristics of 20 amino acids in patients with colorectal cancer(CRC);Constructing a biomarker panel for CRC diagnosis.Methods 100 healthy controls and 113 CRC patients were enrolled(27 in stage Ⅰ,28 in stage Ⅱ,34 in stage Ⅲ and 24 in stage Ⅳ).Targeted quantitative analysis of 20 common amino acids in urine was performed using multiple reaction monitoring technology based on ultra-high performance liq-uid chromatography tandem mass spectrometry(UPLC-MS/MS).Statistical methods were used to analyze the differ-ences in amino acids content between CRC patients and healthy controls,and a CRC diagnostic model was constructed.Results There was a statistical significance in the content of 16 amino acids in CRC urine compared to healthy con-trols,and 9,15,14,and 17 amino acids in Stage Ⅰ,Ⅱ,Ⅲ and Ⅳ showed a statistical difference.A biomarker panel composed of six amino acids,isoleucine,asparagine,proline,histidine,tyrosine,and phenylalanine was constructed for CRC diagnosis.The AUC in the discovery/validation group were 0.857/0.851,and in the Stage Ⅰ/Ⅱ/Ⅲ/Ⅳ of CRC,the AUC value was 0.847/0.843/0.897/0.896,respectively.Conclusion Changes in the content of 20 amino acids in urine can be used for the diagnosis of CRC,and this study provides new research ideas for the diagnosis of CRC.
amino acidsurinetargeted analysiscolorectal cancer
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NSTL
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