Objective To study the plasmid on which the carbapenemase gene is located in the Klebsiella pneumoni-ae QL5 strain and its characteristics.Methods The genome and plasmid sequences of the strain were obtained based on the second-generation Illumina and third-generation Nanopore sequencing technology platforms,and the bioinformatics of the strains was analyzed with a series of software.The plasmid characteristics were detected by S1 nuclease digestion pulse field gel electrophoresis,plasmid conjugation assay and stability testing.Results Bioinformatics showed that blaNDM-1 and blaKpC-2 genes carried by Klebsiella pneumoniae QL5 strains were located on the same IncC/IncFⅡ(PHN7A8)/IncR hybrid plasmid(named pQL5-NDM-KPC)and plasmid stability testing indicated that there was a fragment loss in the region of the plasmid pQL5-NDM-KPC,where blaNDM-1 gene is located.Conclusion To the best of our knowledge,it is firstly reported that the blaNDM-1 and blaKPC-2 genes were located in the same IncC/IncFⅡ(PHN7A8)/IncR hybrid plasmid pQL5-NDM-KPC(GenBank Accession Number:OR253888).
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