ST11肺炎克雷伯菌QL5株携带的质粒pQL5-NDM-KPC的研究
Study on plasmid pQL5-NDM-KPC carried by ST11 Klebsiella pneumoniae QL5 strain
马超越 1王笑 2刘真真 2贾楠 2朱元祺2
作者信息
- 1. 青岛大学附属医院,山东青岛 266003;枣庄市山亭区人民医院,山东枣庄 277200
- 2. 青岛大学附属医院,山东青岛 266003
- 折叠
摘要
目的 探讨肺炎克雷伯菌QL5株携带编码碳青霉烯酶基因的质粒及其特性.方法 基于二代Illumina和三代Nanopore测序技术平台获得菌株的基因组和质粒序列,然后用一系列软件分析菌株的生物学信息.S1核酸酶切脉冲场凝胶电泳、质粒液相接合试验和稳定性试验检测菌株携带的质粒特性.结果 生信分析显示:肺炎克雷伯菌QL5株携带的blaNDM-1和blaKPC-2基因位于同一个IncC/IncFⅡ(PHN7 A8)/IncR杂合质粒上(命名为pQL5-NDM-KPC);QL5株携带的pQL5-NDM-KPC可发生blaNDM-1基因所在区域的片段丢失.结论 经检索,携带blaNDM-1和blaKPC-2基因位于同一个 IncC/IncFⅡ(PHN7A8)/IncR 杂合质粒 pQL5-NDM-KPC(GenBank 序列号:OR253888)上,为国内外首次报道.
Abstract
Objective To study the plasmid on which the carbapenemase gene is located in the Klebsiella pneumoni-ae QL5 strain and its characteristics.Methods The genome and plasmid sequences of the strain were obtained based on the second-generation Illumina and third-generation Nanopore sequencing technology platforms,and the bioinformatics of the strains was analyzed with a series of software.The plasmid characteristics were detected by S1 nuclease digestion pulse field gel electrophoresis,plasmid conjugation assay and stability testing.Results Bioinformatics showed that blaNDM-1 and blaKpC-2 genes carried by Klebsiella pneumoniae QL5 strains were located on the same IncC/IncFⅡ(PHN7A8)/IncR hybrid plasmid(named pQL5-NDM-KPC)and plasmid stability testing indicated that there was a fragment loss in the region of the plasmid pQL5-NDM-KPC,where blaNDM-1 gene is located.Conclusion To the best of our knowledge,it is firstly reported that the blaNDM-1 and blaKPC-2 genes were located in the same IncC/IncFⅡ(PHN7A8)/IncR hybrid plasmid pQL5-NDM-KPC(GenBank Accession Number:OR253888).
关键词
肺炎克雷伯菌/bla/KPC-2/blaNDM-1/质粒Key words
Klebsiella pneumoniae/blaKPC-2/blaNDM-1/plasmid引用本文复制引用
基金项目
青大附院"临床医学+X"科研项目(2019-3399)
出版年
2024
NETL
NSTL
万方数据