肠道菌群代谢物TMAO对兔动脉粥样硬化斑块形成及狭窄程度的影响
Effects of intestinal flora metabolite TMAO on atherosclerotic plaque formation and stenosis in rabbits
路怀志 1池尧 2赵辉 1许学升1
作者信息
- 1. 商丘市第一人民医院心血管内科,河南 商丘 476000
- 2. 吉林大学第二医院心内科,吉林 长春 130041
- 折叠
摘要
目的 探讨氧化三甲胺(Trimetlylamine oxide,TMAO)在兔动脉粥样硬化斑块产生及狭窄程度中的作用.方法 3月龄新西兰雄性白兔32只,随机分为对照组、模型组、TMAO组和TMAO抑制组,每组8只.对照组给予普通饲料喂养,余各组给予高脂饮食.喂养1周后,模型组给予高脂饮食+低温气体损伤兔颈动脉内膜建立动脉粥样硬化斑块模型;TMAO组操作同模型组,并给予氯化胆碱灌胃;TMAO抑制组操作同TMAO组,但饮水中加入TMAO抑制剂3,3-二甲基-1-丁醇(DMB).术后12周再次检测血脂、TMAO等血清学指标,取靶血管样本行HE、Masson、弹力纤维、油红O染色,对比观察斑块形态,分析斑块体积及管腔狭窄程度.结果 喂养13周后高脂饮食各组间LDL-C、HDL-C、TC、TG差异无统计学意义(P＞0.05),而TMAO组血清TMAO水平较余三组均显著升高,差异有统计学意义(P＜0.05).对照组未见明显斑块形成,TMAO组斑块内泡沫细胞丰富,可见较大脂质核心,纤维帽薄,其斑块大小及狭窄率均显著高于模型组及TMAO抑制组,差异有统计学意义(P＜0.05).结论 肠道菌群代谢产物TMAO对血脂无明显影响,但能促进动脉粥样硬化斑块的产生,并加重斑块内脂质沉积,增加狭窄程度.
Abstract
Objective To explore the role of Trimetlylamine oxide(TMAO)in the formation and stenosis of athero-sclerotic plaques in rabbits.Methods 32 3-month-old New Zealand male white rabbits were randomly divided into con-trol group,model group,TMAO group and TMAO inhibition group,with 8 rabbits in each group.The control group was fed with normal feed,and the other groups were fed with high-fat diet.After feeding for 1 week,the model group was given a high-fat diet+low-temperature gas to damage the carotid artery intima of rabbits to establish an athero-sclerotic plaque model.The TMAO group was operated in the same way as the model group,and choline chloride was administered by gavage.The TMAO inhibition group was operated in the same way as the TMAO group,but the TMAO inhibitor 3,3-dimethyl-1-butanol(DMB)was added to the drinking water.Serological indicators such as blood lipids and TMAO were tested again 12 weeks after the operation,and target blood vessel samples were taken for HE,Masson,elastic fiber,and Oil Red O staining to compare the plaque morphology and analyze the plaque volume and lu-men stenosis.Results After 13 weeks of feeding on the high-fat diet,there was no significant difference in LDL-C,HDL-C,TC,and TG among the various groups(P＞0.05).However,the serum TMAO level of the TMAO group was significantly higher than that of the other three groups(P＜0.05).No obvious plaque formation was seen in the control group.The plaques in the TMAO group were rich in foam cells,with larger lipid cores and thin fibrous caps.The plaque size and stenosis rate were significantly higher than those in the model group and the TMAO inhibition group(P＜0.05).Conclusion The intestinal flora metabolite TMAO has no significant effect on blood lipids,but can promote the formation of atherosclerotic plaques,aggravate lipid deposition in plaques,and increase the degree of stenosis.
关键词
氧化三甲胺/易损斑块/急性冠脉综合征/肠道菌群Key words
TMAO/vulnerable plaque/acute coronary syndrome/intestinal flora引用本文复制引用
基金项目
商丘市科技发展指导性计划(20220312)
出版年
2024
NETL
NSTL
万方数据