Objective To study the characteristics of pathogenic bacteria in children with acute respiratory tract in-fection,and to explore the diagnostic value of real-time PCR detection of bronchoalveolar lavage fluid samples from chil-dren with acute lower respiratory tract infections.Methods A total of 172 hospitalized children with acute respiratory tract infection admitted to the First Hospital of Jilin University from June 2018 to May 2019 were selected as the study objects.Bronchoalveolar lavage fluid was collected and pathogens were detected by multiple real-time PCR.Results In this study,106 Bronchoalveolar lavage samples were detected,the positive rate was 61.63%(106172),the top three pathogens were Streptococcus pneumoniae 26.74%(46172),Haemophilus influenzae 17.44%(30172),the detection rate of bordetella pertussis was 8.14%(14/172),and there was difference among different age groups(P<0.01).Con-clusion The detection rate of Streptococcus pneumoniae was the highest in children with acute lower respiratory tract infection,followed by Haemophilus influenzae,bordetella pertussis and Staphylococcus aureus.The detection rate of pathogenic bacteria in children with acute lower respiratory tract infection was different in different age groups.