Objective To investigate the expression pattern of peroxisome proliferator-activated receptor gamma co-activator-1 alpha(PGC1α)in bladder cancer and its impact on the invasiveness of bladder cancer cells.Methods The differential expression of the PGC1α gene in serum samples from bladder cancer patients and control subjects(no n-blad-der cancer patients)was analyzed,and the relationship between the expression level of PGC1α and the clinical stage and pathological grade of bladder cancer was examined.PGC1α overexpression experiments were conducted in the bladder cancer cell line T24 to analyze its effects on cell proliferation,colony formation,and invasion.Western blot experiments were performed to detect the expression of Epithelial-Mesenchymal Transition(EMT)-related proteins.Results The expression level of PGC1α in serum samples from bladder cancer patients was significantly lower than that in the con-trol group,and bladder cancer patients with high PGC1α expression had lower clinical stages and pathological grades.Overexpression of PGC1α resulted in significantly decreased proliferation,colony formation,and invasion abilities of T24 cells.Furthermore,PGC1α overexpression led to downregulation of EMT-related proteins SNAIL1,N-cadherin,and Vimentin,as well as upregulation of E-cadherin expression.Conclusion(1)Decreased expression of PGC1α is an important factor in the development of bladder cancer and is associated with higher clinical stages and pathological grades.(2)PGC1α can inhibit tumor cell proliferation and invasion by regulating EMT-related proteins.
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