Expression of HSP90α in cervical cancer cells and tissues and its relationship with tumor apoptosis
Objective To investigate the expression of HSP90α in cervical cancer cells and tissues and its relation-ship with tumor apoptosis.Methods 103 patients with cervical cancer admitted to our hospital from April 2019 to A-pril 2021 were selected,and the cancerous and adjacent tissues were removed during surgery.The relative expression of HSP 90α mRNA in the tissues was detected by RT-qPCR method,and the relationship between the expression of HSP 90α gene and the clinical pathological features and progression-free survival of the patients was analyzed.RT-qPCR was used to detect the expression level of HSP90α in HeLa,CaSki,C-33A cervical cancer cells and normal cervical epithelial cells End1/E6E7.Using HeLa cells,they were divided into blank control group,shRNA group,and sh-NC group,and the expression levels of HSP90α mRNA in each group of cells were detected.The proliferation of each group of cells was detected by CCK8 method,and the apoptosis of each group of cells was detected by flow cytometry.The expression of apo ptosis-related genes in each group of cells was detected by Western Blot method.Results The relative expression of HSP 90α mRNA in cervical cancer tissues was significantly higher than that in adjacent tissues(P＜0.05).The ex-pression of HSP90α mRNA in cancer tissue is related to the depth of invasion,clinical stage,and lymph node metastasis of the patients(P＜0.05).The progression-free survival of patients with low expression of HSP90α was significantly better than that of patients with high expression(P＜0.05).The relative expression levels of HSPα mRNA in cervical cancer cell lines HeLa,CaSki,and C-33A were significantly higher than those in normal cervical epithelial cell line End1/E6E7(P＜0.05).The relative expression of HSP90α mRNA in shRNA group was significantly lower than that in the blank control group and sh-NC group(P＜0.05).At 24 h,48 h,72 h,and 96 h,the absorbance at 450nm in the shRNA group was significantly lower than that of the blank control group and the sh-NC group(P＜0.05).The cell apoptosis rate in the shRNA group was significantly higher than that in the blank control group and the sh-NC group(P＜0.05).In the shRNA group,the relative expression levels of Caspase-3 and Bax proteins were significantly higher than those in the blank control group and the sh-NC group(P＜0.05),while the relative expression levels of Bcl-2 and Cyclin D1 proteins were significantly lower than those in the blank control group and the sh-NC group(P＜0.05).Con-clusion High expression of HSP90α is found in cervical cancer tissues and cells.HSP90α is involved in the develop-ment and progression of cervical cancer.Inhibiting HSP90α expression in cervical cancer cells can effectively inhibit tumor proliferation activity,activate the cell apoptosis pathway and promote cell apoptosis.HSP90α has the potential to be a biomarker for cervical cancer treatment.
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