Study on the mechanism of Meihuacao extracts regulating gut microbiota to suppress primary glioma models
Objective To investigate the effect of Meihuacao on intestinal microbiota of bearing mice during glioma development.Methods The 4-week-old Balb/c female nude mice were intracranially implanted with either U87-MG or RFP-U87-MG gliomas,the former for intestinal flora assay(6 per group,divided into 2 groups),and the latter for in vi-vo fluorescence imaging to evaluate the growth status of gliomas(3 per group,divided into 2 groups).Meihuacao ex-tracts were administered by gavage(25 mg/kg)every other day for 10 consecutive times(from day 6 to day 24 after im-plantation),and the control group was 1*PBS.The RFP-U87-MG model mice were subjected to in vivo fluorescence imaging to observe the tumor growth status on days 14 and 24,while nude mice were weighed,and after euthanasia of nude mice on day 25,the brain tissues and tumors were exfoliated and subjected to separate weighing following HE staining of these tissues.While the U87-MG model mice were weighed each 3 days,fresh fecal specimens were collected from the colon by euthanasia on the 25th day,and the gut microbes was analyzed by Illimina high-throughput sequen-cing for bioinformatics mining of characteristic species potentially related to the treatment of Meihuacao.Results In vivo fluorescence imaging and weighing of tumor foci suggested that Meihuacao extracts significantly inhibited the ma-lignant proliferation of primary gliomas,and HE staining exhibited that the number of tumor cells in the Meihuacao group was reduced,the nuclear-to-plasm a ratio tended to be normalized,and the nuclear anisotropy was reduced.After high-throughput sequencing,it was found that the intervention of Meihuacao extracts did not affect the α-diversity of the intestinal flora,but rather the abundance of the characteristic microbes,in which the treatment of Meihuacao ex-tracts could significantly increase Muribaculum intestinale,Bacteroides faecichinchillae,Klebsiella oxytoca,while de-creasing the abundance of Lachnospiraceae bacterium A4 and Helicobacter japonicus.Conclusion While significantly inhibiting the malignant proliferation of in situ gliomas,Meihuacao extracts affected the abundance of gut microbiota,in which the characteristic microbes,such as Muribaculum intestinale and Bacteroides faecichinchillae,increased,which may be involved in the mechanistic regulation of brain tumors as probiotics,and also provides a novel research method for the use of Meihuacao as a potential prebiotic mediating gut-microecology-brain axis with the secretion of related transmitters to inhibit brain tumors.
万方数据
维普
