Objective To explore the migration effect of NF-κB inhibitor APDC on human skin basal cell carcinoma and its mechanism.Methods To detect of the effect of NF-κB inhibitor APDC on the proliferation activity of human BCC cells using MTT assay.Observation of changes in the growth characteristics of BCC cells after the application of NF-κB inhibitor through cell scratch and Transwell invasion experiment,Application of RT-qPCR to detect the effect of different concentrations of APDC inhibitor on NF-κB mRNA expression in TE354.T cells,finally,the impact on protein expression was explored through Western blot.Results NF-κB inhibitor APDC has an inhibitory effect on the prolifer-ation of TE354.T cells in a dose-dependent manner,APDC effectively inhibits the migration and invasion ability of TE354.T cells in a dose-dependent manner over time,at the same time,APDC has an inhibitory effect on the mRNA level of TE354.T cells,showing a positive correlation and reducing protein expression.Conclusion NF-κB inhibitor APDC can inhibit cell proliferation of BCC,affect the expression of BCC NF-κB protein and mRNA levels,and effective-ly inhibit the migration and invasion ability of skin basal cell carcinoma.
关键词
NF-κB/皮肤基底细胞癌/细胞增殖/细胞迁移/细胞侵袭
Key words
NF-κB/basal cell carcinoma of the skin/cell proliferation/cell migration/cell invasion
维普
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