Lipid metabolism disorders destroy mitochondrial function and lead to atherosclerosis in rats
Objective To investigate the role of mRNA expression level of lipid metabolism related genes and mito-chondrial function in the formation of aortic atherosclerosis through a high-fat diet model in rats.Methods The athero-sclerotic model of rats was established by high-fat diet combined with vitamin D3.Serum lipid indexes and mRNA ex-pression levels of lipid metabolism genes and mitochondrial synthesis and decomposition genes were detected.Results Atherosclerosis of aorta and carotid artery in the model group was obvious,and the lesion aggravated with time.Com-pared with the control group,the levels of Lp(a),TC,TG,and LDL-C in the model group were significantly higher than those in the control group(P＜0.05),while there was no statistical difference in apoB 100 and HDL-C(P＞0.05).In addition,the mRNA expression levels of lipid metabolism related genes SREBF2,LDL,HMGCR,FDFT1,HMGCS 1,LPL,and DHCR24,as well as mitochondrial synthesis and decomposition genes MFN2 and Drp1,were significantly increased in the model group(P＜0.05),while there was no significant change in the mRNA expression levels of ABCA and SQLE(P＞0.05).Conclusion The key to atherosclerosis is the increase of blood lipid caused by high-fat diet.The mRNA expression level of lipid metabolism genes in aortic lesion tissues is significantly increased,and mitochondrial synthesis and decomposition are significantly increased.
万方数据
维普
