To establish a mouse diarrhea model caused by porcine rotavirus(PoRV)OSU strain and investigate its pathogenicity in neonatal mice,forty 4-day-old Kunming mice were used in this study.The mice were randomly divided into three infected groups and one control group.The infected groups were orally administered different doses of PoRV culture fluid,with doses of Infection group 1(50 μL),Infection group 2(100 μL),and Infection group 3(150 pL),with a viral titer of 105.2 TCID50/100 μL.The Control group was orally administered cell culture fluid using the same method.Clinical symptoms,diarrhea,mortality,and autopsy findings of the neonatal mice were observed at different time points after infection.Histopathological examination of intestinal tissues was conducted using hematoxylin eosin(H.E.)staining,and the PoRV content in the intestinal contents was detected using reverse transcription-polymerase chain reaction(RT-PCR).The results showed that neonatal mice exhibited significant diarrhea symptoms at 24 hours after PoRV inoculation.The most severe symptoms occurred on days 3 to 4 post-infection,with diarrhea rates ranging from 62.5％to 100％.By day 7,the neonatal mice had recovered to normal,with mortality rates ranging from 44.4％to 90.0％.Compared to the Control group,mice in the infected groups exhibited pronounced pathological changes in the intestines,mainly characterized by edema,congestion,and disruption of intestinal epithelial integrity.RT-PCR results showed the presence of PoRV in the intestinal contents of some mice in the infected groups.This study successfully established a mouse diarrhea model using the PoRV OSU strain and elucidated its pathogenic characteristics.
维普
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