Histopathological Diagnosis and Immunohistochemical Analysis of Canine Extramedullary Plasmacytoma
Extramedullary plasmacytoma(EMP)is a relatively rare tumor in dogs.To investigate the histopathological and immunohistochemical characteristics of canine EMP,this study analyzed skin tumor samples from affected dogs using hematoxylin-eosin(H.E.)staining and Congo red staining for histopathological diagnosis.Immunohistochemical(IHC)staining was further performed to examine the expression of EMP-specific markers,including paired box gene 5(PAX5),cluster of differentiation 20(CD20),cluster of differentiation 79a(CD79a),multiple myeloma oncogene 1(MUM1),and canine immunoglobulin G(Canine IgG).The results showed that the tumors in both cases lacked encapsulation but were well-demarcated from surrounding tissues.Tumor cells exhibited plasmacytoid or plasmablastic characteristics,and amyloid deposition was observed in the tumor tissue of Case 2.Immunohistochemically,the tumor cells in Case 1 were negative for PAX5,CD20,and MUM1,but positive for CD79a and Canine IgG.In Case 2,the tumor cells were negative for PAX5,but positive for CD20,CD79a,MUM1,and Canine IgG.These findings confirmed that both cases were cutaneous forms of canine EMP based on histopathological and IHC results.This study provides significant pathological evidence for the accurate diagnosis of canine EMP,serving as an important reference for clinical and research purposes.
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