Serological Investigation and Analysis of Avian Leukosis Subgroups A,B and J in Main Wenchang Chicken Breeding Area in Hainan Province
To understand the infection status of avian leukosis virus(ALV)subgroups A,B,and J in Wenchang chickens in Hainan Province,1 746 serum samples were randomly collected from Wenchang chicken farms in key breeding areas(Haikou,Wenchang,Danzhou,Qionghai,and Chengmai)between December 2021 and December 2022.Enzyme linked immunosorbent assay(ELISA)was used to detect ALV-J and ALV-A/B antibodies,and the antibody-positive rates were statistically analyzed based on region,growth stage,season,farm size,and chicken type.The results showed that among the 1 746 serum samples,182 tested positive for ALV-J antibodies,with an overall antibody positive rate of 10.42%(95%CI:8.99%-11.86%),and five samples tested positive for ALV-A/B antibodies,with a total antibody positive rate of 0.29%(95%CI:0.04%-0.54%).ALV-J infection was present to varying degrees in all five regions,with the highest antibody positive rate in Danzhou at 50.00%(30/60)(95%CI:37.24%-62.76%).ALV-A/B antibody positive samples were found only in Haikou,with a positive rate of 0.49%(5/1 011)(95%CI:0.06%-0.93%).The ALV-J antibody positivity rate in adult chickens was significantly higher than that in growing chickens and chicks(P<0.05),reaching 14.60%(138/945)(95%CI:12.35%-16.86%).ALV-J infections occurred year-round,peaking in winter with a positive rate of 23.12%(86/372)(95%CI:18.83%-27.41%),while ALV-A/B was only detected in summer and autumn.Small-and medium-scale farms showed significantly higher ALV-J antibody-positive rates than large-scale farms(P<0.05),at 20.42%(88/431)(95%CI:16.61%-24.23%)and 21.48%(29/135)(95%CI:14.52%-28.44%),respectively.Commercial broilers had the highest ALV-J antibody-positive rate of 20.13%(61/303)(95%CI:15.61%-24.65%).These findings indicate that ALV-J infection is prevalent in Wenchang chicken breeding areas in Hainan,while ALV-A/B occurs sporadically only in Haikou.ALV-J infection is more common in winter and among adult Wenchang chickens,with commercial broilers being the most affected.Targeted measures should be implemented to strengthen the control of ALV-J in Hainan's Wenchang chicken population.
avian leukosissubgroups A,B and Jserological investigationWenchang chickenHainan Province
万方数据
维普
