2016-2023年上海市浦东新区和松江区畜禽源大肠埃希菌耐药性比较
Comparison of Drug Resistance of Escherichia coli Isolated from Livestock and Poultry in Pudong New Area and Songjiang District of Shanghai from 2016 to 2023
方雅红 1张妤 1孙冰清 1姜芹 1龚志亮 2李闽 3商军 1张亦菲1
作者信息
- 1. 上海市动物疫病预防控制中心,上海 长宁 201103
- 2. 上海市浦东新区畜牧水产技术推广中心,上海 浦东 201299
- 3. 上海市松江区动物疫病预防控制中心,上海 松江 201699
- 折叠
摘要
为了调查2016-2023年上海市浦东新区和松江区(下文简称"两区")畜禽源大肠埃希菌(E.coli)的耐药性变迁情况,本试验从两区畜禽养殖场分别采集401和490份畜禽肛拭子进行大肠埃希菌的分离鉴定,并调查养殖场的抗菌药物使用情况;采用微量肉汤稀释法测定分离的大肠埃希菌对10大类14种抗菌药物的敏感性.结果显示,两区不同养殖场的抗菌药物使用情况存在差异.两区分别分离到333和350株大肠埃希菌,分离率为83.04%和71.43%.两区分离菌株对四环素(87.09%,82.00%)、磺胺异噁唑(77.78%,82.00%)、氨苄西林(82.58%,70.30%)和复方新诺明(70.87%,76.90%)的耐药率较高,对头孢他啶(6.61%,6.90%)和黏菌素(0.90%,0.90%)较为敏感,未检测到美罗培南耐药菌株;多重耐药菌株占比分别为87.99%和81.71%.8年间,两区分离菌株对多数抗菌药物的耐药率、多重耐药菌株占比和耐药谱数量整体呈下降趋势,松江区下降趋势更明显.结果表明,浦东新区和松江区畜禽源大肠埃希菌对部分抗菌药物的耐药率高且多重耐药情况严重,在减抗背景下,两区耐药水平均有缓解,松江区缓解程度更大.
Abstract
To investigate the trends in drug resistance of Escherichia coli(E.coli)from livestock and poultry in Pudong New Area and Songjiang Districts of Shanghai(hereafter referred to as the"two districts")from 2016 to 2023,this study collected 401 and 490 anal swab samples from livestock and poultry farms in the two districts,respectively.E.coli isolates were identified,and the antimicrobial usage on these farms was surveyed.The drug susceptibility of the isolated E.coli strains against 14 antimicrobial agents across 10 classes was determined using the broth microdilution method.The results showed that antimicrobial usage varied across farms in the two districts.A total of 333 and 350 E.coli isolates were obtained from the two districts,with isolation rates of 83.04%and 71.43%,respectively.High resistance rates were observed for tetracycline(87.09%,82.00%),sulfisoxazole(77.78%,82.00%),ampicillin(82.58%,70.30%),and trimethoprim/sulfamethoxazole(70.87%,76.90%).Low resistance rates were found for ceftazidime(6.61%,6.90%)and colistin(0.90%,0.90%),and no resistance to meropenem was detected.Multidrug-resistant strains accounted for 87.99%and 81.71%of isolates,respectively.Over the eight years,the overall resistance rates,proportion of multidrug-resistant strains,and resistance spectrum breadth showed a declining trend,with a more significant reduction in Songjiang District.These findings indicate that E.coli from livestock and poultry in Pudong New Area and Songjiang Districts exhibit high resistance rates to certain antimicrobials and severe multidrug-resistant issues.However,under the framework of antimicrobial reduction policies,the drug resistance levels have shown improvement,with Songjiang District experiencing a more substantial decrease.
关键词
大肠埃希菌/耐药性/兽用抗菌药/减量化行动/浦东新区/松江区Key words
Escherichia coli/drug resistance/veterinary antimicrobial drugs/reduction action/Pudong New Area/Songji-ang District引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据