Serological Epidemiological Investigation and Analysis of Canine Leptospira in Shanghai from 2020 to 2023
This study aimed to understand the immune and clinical infection status of canine Leptospira in Shanghai,identify the dominant serogroups carried by dogs,and provide critical data support for the prevention and control of canine leptospirosis in the region.A total of 3 920 canine serum samples(3 557 from stray dogs,176 from vaccinated domestic dogs,and 187 from unvaccinated domestic dogs)collected between 2020 and 2023 were tested using enzyme-linked immunosorbent assay(ELISA).For ELISA-positive samples(n=80),microscopic agglutination test(MAT)was conducted to identify the Leptospira serogroups,followed by statistical analysis of seropositivity rates and serogroup data.The results showed that the seropositivity rate of Leptospira in stray dogs was 52.38%(95%CI:51.22%-53.54%),while that in domestic dogs was 19.28%(95%CI:15.22%-23.34%).The serogroup analysis revealed that in stray dogs,the predominant serogroups were Canicola>Australis>Icterohaemorrhagiae>Balum>40%,with no detection of Grippotyphosa.In domestic dogs,all 15 serogroups were detected;in unvaccinated domestic dogs,the predominant serogroups were Icterohaemorrhagiae>Autumnalis=Sejroe>Canicola>50%,while in vaccinated domestic dogs,the predominant serogroups were Balum>Pamonoa>Pyrogenes>50%(vaccine-related serogroups Icterohaemorrhagiae and Canicola were excluded from dominance comparison).Statistical analysis revealed regional differences in the number of infecting serogroups in unvaccinated domestic dogs(P<0.05).Significant differences in seropositivity rates and serogroup types(Australis,Pamonoa,Grippotyphosa,and Tarassovi)were observed between stray and domestic dogs(P<0.05).Additionally,significant differences in serogroup types(Balum,Pyrogenes,and Autumnalis)were found between vaccinated and unvaccinated domestic dogs(P<0.05).The findings suggest that the dominant Leptospira serogroups vary among different canine populations in Shanghai,with a higher seropositivity rate in stray dogs,indicating the potential public health risks posed by stray dogs.
国家科技期刊平台
NSTL
万方数据
