Therapeutic Effect of Vitellogenin 2 on Dexamethasone-Induced Obesity
Vitellogenin 2(VTG2)has been shown to promote the browning process of 3T3-L1 adipocytes,but its potential role in treating obesity by promoting the browning of adipose tissue in vivo has not been reported.This study aims to explore the effects of VTG2 on the browning of white adipose tissue(WAT)in mice.The study involved two groups:the adeno-associated virus(AAV)empty vector group(AAV9-NC1)and the VTG2 treatment group(AAV9-VTG2).Hematoxylin-eosin(H.E.)staining was used to observe the size of WAT adipocytes,while immunohistochemistry(IHC),real-time quantitative polymerase chain reaction(qPCR),and Western blotting(WB)were used to detect the expression of the browning markers uncoupling protein-1(UCP1)and peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC1α)in WAT.To further investigate the effect of VTG2 on dexamethasone(DEX)-induced browning of WAT in obese mice,three groups were established:the AAV empty vector group(AAV9-NC2),the DEX treatment group(AAV9-NC+DEX),and the VTG2 treatment group(AAV9-VTG2+DEX).The same methods were applied to detect the same markers.The results showed that,compared with the AAV9-NC1 group,the AAV9-VTG2 group had significantly smaller inguinal WAT adipocytes(P<0.01),and the mRNA expression levels of UCP1 and PGC1α were significantly higher(P<0.001 or P<0.01),with protein levels also significantly upregulated(P<0.01 or P<0.05).Compared with the AAV9-NC2 group,the AAV9-NC+DEX group had significantly larger inguinal WAT adipocytes(P<0.01),with a marked decrease in the mRNA expression of UCP1 and PGC1α(P<0.001 or P<0.01),and the protein levels were significantly reduced(P<0.001).Furthermore,compared with the AAV9-NC+DEX group,the AAV9-VTG2+DEX group had significantly smaller inguinal WAT adipocytes(P<0.001),and the mRNA expression levels of UCP1 and PGC1α were significantly higher(P<0.001 or P<0.01),with protein levels significantly upregulated(P<0.05).In conclusion,VTG2 promotes the browning of WAT and has a protective effect against DEX-induced obesity,providing new research directions for the treatment of obesity.
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