Waterproofing of tunnel and underground engineering has been a hot issue in the academic and engineering circles for a long time.Taking Guiyang rail transit tunnel and underground engineering as an example,according to the geological conditions and structural characteristics of urban rail transit in karst mountainous areas,this paper expounds in detail the practice of waterproof design and material selection,and comprehensively analyzes the main reasons for the failure of waterproof system in practice.It also introduces the exploration and application of new materials,new technologies and new processes in the waterproof field of tunnel and underground engineering,which can provide reference ideas for the management,design and construction of similar projects.