首页|24份农艺性状优异割手密黑穗病抗性鉴定及Bru1基因分子检测

24份农艺性状优异割手密黑穗病抗性鉴定及Bru1基因分子检测

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[目的]筛选抗黑穗病和褐锈病的割手密种质,为甘蔗黑穗病和褐锈病抗病亲本选育提供理论参考。[方法]采用人工浸渍接种法及与褐锈病基因Bru1 紧密连锁的 2 个分子标记,分别对24 份割手密进行甘蔗黑穗病和褐锈病抗性鉴定。[结果]结果表明,试验的割手密材料表现出较强的抗黑穗病能力,有16 份材料表现1 级高抗,5 份材料表现2 级抗病,3 份材料表现3 级抗病;分子检测结果显示,有7 份材料含有Bru1 基因,占参试材料的 29。17%。[结论]本研究筛选出的高抗黑穗病且兼含Bru1 基因的种质,是培育抗黑穗病和抗褐锈病的甘蔗品种(亲本)的良好抗源材料。
Identification of Resistance to Smut and Molecular Detection of Bru1 Gene in 24 Elite Saccharum spontaneum L.
[Objective]The aim of this study was to screen Saccharum spontaneum L.(S.spontaneum L.)germplasm resistant to smut and brown rust,so as to provide theoretical references for the research on disease-resistant parents for breeding for resistance to smut and brown rust in sugarcane.[Methods]The identification resistance to smut for 24 elite S.spontaneum L.was conducted by the method of dipping inoculation,and the resistance to brown rust was conducted by testing two molecular markers Bru1 closely linked to brown rust gene.[Results]The results showed that the test materials showed strong resistance to smut,16 performed high resistance at level 1,5 materials performed resistance at level 2,and 3 materials performed resistance at level 3;The molecular detection indicated that 7 materials contained the Bru1 gene,accounting for 29.17%of all the tested materials.[Conclusion]The germplasms selected in this study with high resistance to smut and containing Bru1 gene are good sources for developing sugarcane varieties(parents)with resistance to smut and brown rust disease.

Saccharum spontaneum L.smutbrown rustresistance identification

黄玉新、黄冬梅、陆衫羽、颜梅新、高丽花、周珊、杨翠芳、段维兴、张小秋、高轶静、张革民、张保青

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广西壮族自治区农业科学院甘蔗研究所/农业农村部广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室,南宁 530007

割手密 黑穗病 褐锈病 抗性鉴定

广西科技重大专项广西自然科学基金项目广西自然科学基金项目中国科学院华南植物园重点支持项目广西农业科学院基本科研业务专项项目广西农业科学院基本科研业务专项项目广西农业科学院基本科研业务专项项目广西重点实验室建设项目系统性研究课题

桂科AA22117002-12023GXNSFAA0263542023GXNSFAA026088E36101桂农科2021YT0042023YM532024YP02821-238-16-A-01-02

2024

中国糖料
中国农业科学院甜菜研究所

中国糖料

影响因子:0.672
ISSN:1007-2624
年,卷(期):2024.46(2)
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