Cloning and Functional Prediction of the Sugar Beet Cadmium Stress-responsive Gene ZAT10
[Objective]To predict the structural characteristics and functions of sugar beet zinc finger protein BvZAT10(LOC 104901462),[Methods]using sugar beet'780016B/12 superior'as experimental material,BvZAT10 gene was cloned and subjected to bioinformatics analysis,and the transcriptional expression profile data of sugar beet in response to cadmium stress were used to explore the gene response characteristics under cadmium stress.[Results]The coding region of BvZAT10 is 729 bp in length,encoding 242 amino acids.It is an alkaline,unstable hydrophilic protein with two C2H2-type zinc finger domains.The protein encoded by this gene has a total of 36 phosphorylation modification sites,and the protein structure is mainly characterized by irregular coiling.Phylogenetic analysis revealed that BvZAT10 is most closely related to ZAT10 in spinach and quinoa.The BvZAT10 promoter sequence contains multiple functional elements related to plant hormone response,light response,and stress resistance.Under 1,3 and 5 mmol/L CdCl2 treatments,BvZAT10 and most of its predicted regulatory target genes,such as metal tolerance protein 11(BVRB_2g037080)and ABCG family member 22(BVRB_5g109050),were positively regulated by cadmium stress.[Conclusion]It is speculated that BvZAT10 may play an important role directly or indirectly in sugar beet's response to cadmium stress and could be further studied as a potential dominant stress-tolerant gene.
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