Insula is involved in the regulation of headache and related anxiety behaviors rats through GluA1 receptor and adenylate cyclase 1
Objective:This study aims to determine the role of the insula in the pathogenesis of migraine and ex-plore its underlying mechanisms through a rat model.Methods:A chronic migraine(CM)rat model was established by continuous dural infusion of inflammatory soup.The periorbital mechanical threshold was assessed using von Frey filaments.Anxiety behaviors in animals were observed through open field test and elevated plus maze test.The expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid(AMPA)receptor subunit 1-GluA1 receptor and adenylate cyclase 1(AC1)in the insula was detected by western blot test.A potential mechanism was explored by injecting an AC1 inhibitor bilaterally into the insula.Results:Continuous inflammatory soup stimulation of the dura mater could induce migraine-like headache and anxiety behaviors in rats.Compared with the control group,the expression of c-Fos,GluA1,the phosphorylate of GluA1(p-GluA1-S845),and AC1 in the insula of the CM group significantly increased.Injection of the AC1 inhibitor into the insula could alleviate hyperalgesia and anxiety behaviors in rats,and reduce the expression level of AC1,GluA1 and p-GluA1-S845 in the insula.Conclusion:The insula may regulate migraine-like headache and anxiety behaviors in rats through GluA1 and AC1.
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