Tracing dye DiI affects the electrical properties of dorsal root ganglion neurons in ulcerative colitis mice
Objective:To investigate whether the tracer dye DiI affects the excitability of dorsal root ganglion(DRG)neurons in mice in an experiment applying neural tracing combined with patch clamp technique.Methods:Using SPF-grade male C57BL/6J mice,the ulcerative colitis(UC)model was induced by 5%dextran sulfate sodium(DSS).DiI was injected at multiple points in T12-L2 dermatomes to retrogradely label somatosensory neurons.Whole mount DRG were isolated and subjected to whole-cell patch clamp recording.Results:In the HE staining,the structure of colonic mucous glands of model group were destroyed,indicating successful induction of the model.Patch clamp recording of unlabeled animal DRG neurons showed that the excitability of the cell membrane from the mice in DSS group was higher than that from the Con group.In the groups treated with DiI,excitability of DiI+neurons in both the Con+DiI and DSS+DiI groups was lower than that of DiI-neurons in the same group.The excitability of DiI+neurons and DiI-neurons in the DSS+DiI group was lower than that of the corresponding neurons in the Con+DiI group,respectively.Compared with DRG neurons in two untracked groups,the excitability of DiI-neurons in the two tracking groups was increased,respectively.Conclusion:Our results demonstrated that the tracing dye DiI mainly reduces the excitability of dorsal root ganglion neurons,suggesting that the influence of tracer should be avoided in the design of relevant experiments for the reason that it may mask the changes in neuronal excitability caused by animal models.
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