Analgesic mechanism study of neuropathic pain relieved by early spinal cord stimulation in rats with spinal cord injury
Objective:To investigate the efficacy of spinal cord electrical stimulation on neuropathic pain in rats with spinal cord injury and its effects on the expression of transient receptor potential vanilloid 1(TRPV1)and C-C chemokine ligand 2(CCL2)in the spinal cord dorsal horn.Methods:Sixty-four female SD rats were randomly divided into four groups:Naïve group,sham surgery group,spinal cord injury group,and spinal cord injury+electrical stimulation group,with 16 rats in each group.Changes in paw mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were observed before surgery,7 days post-surgery,and 14 days post-surgery.TRPV1,CCL2 mRNA and protein expression were detected with qPCR and Western Blot,respectively,and the levels of inflammatory cytokines IL-6,IL-1β,and TNF-α were measured with ELISA.Results:Compared to the control and sham surgery groups,rats in the spinal cord injury group showed a decrease in 50%MWT and TWL at 7 and 14 days post-surgery,enhanced transcription and expression of TRPV1 and CCL2(P<0.05),and increased expression of IL-6,IL-1β,and TNF-α(P<0.001).Comparison with the spinal cord injury group,the spinal cord injury+electrical stimulation group exhibited an increase in 50%MWT and TWL,a reduction in transcription and expression of TRPV1 and CCL2(P<0.05),and a decrease in the expression of IL-6,IL-1β,and TNF-α(P<0.05).Conclusion:Spinal cord electrical stimulation may alleviate neuropathic pain after spinal cord injury by inhibiting the expression of TRPV1 and CCL2 and mitigating the inflammatory response.
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