Study on the mechanism of silver needle therapy in myofascial pain syndrome based on nontargeted metabolomics
Objective:To investigate the mechanism of silver needle therapy in the treatment of myofascial pain syndrome (MPS) rats by non-targeted metabolomics. Methods:Local blunt blow combined with eccentric exercise were employed to established MPS rat model,Mechanical withdrawal threshold (MWT) was detected during modeling and treatment,the pathological morphological changes of local muscle tissue were observed by hematoxylin-eosin staining. The metabolic profiling of localized muscle tissues was performed by ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (LC-MS/MS),quantitative identification was performed by mzCloud,mzVault,and Masslist database comparison,and metabolites were annotated by KEGG,HMDB,and LIPIDMaps databases. Principal component analysis (PCA) and partial least squares discrimination analysis (PLS-DA) were used for multivariate statistical analysis and differential metabolite screening. The metabolic pathways related to the metabolites were enriched by KEGG database to explore the silver needle therapy for the treatment of MPS possible mechanisms. Results:Compared with the model group,the MWT in the treatment group was significantly reduced,and the pathological morphology improved significantly. Statistical analysis of LC-MS/MS assay revealed the local muscle tissue metabolic spectrum of MPS rats was significantly changed after silver needle therapy,and a total of 40 differential metabolites were statistically screened in the positive and negative ion mode,in which 10 differential metabolites were involved in the enriched metabolic pathway,The metabolic pathways related to silver needle heat conduction treatment of MPS rats might include choline metabolism,glycerophospholipid metabolism,and tryptophan metabolism,in which the main differential metabolites involved include LPC 20∶0,LPC 16∶1,LPC 16∶0,L-kynurenine,indole,and 3-indoleacetonitrile. Conclusion:The mechanism of silver needle therapy in the treatment of MPS involves local muscle tissue choline metabolism,glycerophospholipid metabolism,and tryptophan metabolism.
万方数据
维普
