摘要
目的:探讨党参炔苷(LOB)调节音猬因子(Shh)/胶质细胞瘤转录因子1(Gli1)信号通路对胃癌细胞增殖、凋亡和上皮间质转化(EMT)的影响.方法:体外培养MKN-45细胞,分为对照组、LOB组(10μmol/L LOB)、SHH 特异性激活剂组(PM 组,1 μmol/L PM)、LOB+PM 组(10 μmol/L LOB+1 μmol/L PM);CCK-8实验检测MKN-45细胞增殖;划痕试验检测MKN-45细胞迁移;Transwell检测MKN-45细胞侵袭;流式细胞术检测MKN-45细胞凋亡;Western blot检测EMT相关蛋白和Shh、Gli1蛋白表达水平.结果:相较于对照组,LOB组吸光度(A450)值、划痕愈合率、细胞侵袭数目及Vi-mentin、Gli1、Shh、N-cadherin蛋白表达下降(P＜0.05),细胞凋亡率和E-cadherin蛋白表达显著升高(P＜0.05);PM组A450值、划痕愈合率、细胞侵袭数目及Vimentin、Gli1、Shh、N-cadherin蛋白表达增高(P＜0.05),细胞凋亡率和E-cadherin蛋白表达显著降低(P＜0.05);与LOB组相比,LOB+PM组A450值、划痕愈合率、细胞侵袭数目及Vimentin、Gli1、Shh、N-cadherin蛋白表达增高(P＜0.05),细胞凋亡率和E-cadherin蛋白表达显著降低(P＜0.05).结论:LOB可诱导MKN-45细胞凋亡,抑制增殖和EMT,这可能与调节Shh/Gli1信号通路有关.
Abstract
Objective:To investigate the effects of lobetyolin(LOB)on proliferation,apoptosis,and epithelial mesenchymal transition(EMT)of gastric cancer cells by regulating the sonic hedgehog(Shh)/glioma associated oncogene homolog 1(Gli1)signaling pathway.Methods:MKN-45 cells were cultured in vitro and separated into control group,LOB group(10 μmol/L LOB),SHH specific activator group(PM group,1 μmol/L PM),and LOB+PM group(10 μmol/L LOB+1 μmol/L PM);CCK-8 experiment was applied to detect the proliferation of MKN-45 cells;scratch test was applied to detect the migration of MKN-45 cells;Transwell was applied to detect the invasion of MKN-45 cells;flow cytometry was applied to detect the apoptosis of MKN-45 cells;Western blot was ap-plied to detect the expression level of EMT-related proteins and Shh,Gli1 proteins.Results:Com-pared with the control group,the absorbance(A450)value,scratch healing rate,number of cell inva-sions,and the expression of Vimentin,Gli 1,Shh and N-cadherin proteins in the LOB group were re-duced(P＜0.05),the apoptosis rate and the expression of E-cadherin protein were increased(P＜0.05);the absorbance(A450)value,scratch healing rate,number of cell invasions,and the expression of Vi-mentin,Gli1,Shh and N-cadherin proteins in the PM group were increased(P＜0.05),the apoptosis rate and the protein expression of E-cadherin were reduced(P＜0.05).Compared with the LOB group,the absorbance(A450)value,scratch healing rate,number of cell invasions,and the expression of Vimentin,Gli1,Shh and N-cadherin proteins in the LOB+PM group were increased(P＜0.05),the apoptosis rate and the expression of E-cadherin protein were reduced(P＜0.05).Conclusion:LOB may inhibit proliferation and EMT of MKN-45 cells and induce apoptosis by regulating the Shh/Gli1 signaling pathway.
NETL
NSTL
维普
万方数据