摘要
目的:探究RNF113A对肝癌细胞增殖、迁移、侵袭、凋亡和自噬的影响.方法:肝癌细胞Hep3B分成control组和RNF113A过表达组,HepG2分成control组和RNF113A敲低组,CCK-8实验检测细胞活力的变化,克隆形成实验检测细胞增殖能力的变化,Transwell实验检测细胞侵袭能力的变化,划痕实验检测细胞迁移能力的变化,流式细胞术检测细胞凋亡能力的变化,Western blot实验检测自噬相关基因和AMPK信号通路相关基因的蛋白表达变化.结果:与control组相比,RNF113A过表达组中Hep3B细胞的增殖、克隆、侵袭和迁移能力均提高,而凋亡和自噬能力均下降,AMPK信号通路受抑;而RNF113A敲低组中HepG2细胞的增殖、克隆、侵袭和迁移能力显著下降,凋亡和自噬能力升高,促进AMPK信号通路的激活.结论:RNF113A通过抑制AMPK信号通路促进肝癌细胞的增殖、克隆、侵袭和迁移,并抑制凋亡和自噬.
Abstract
Objective:To explore the effects of RNF113A on the proliferation,migration,in-vasion,apoptosis,and autophagy of hepatocellular carcinoma cells.Methods:Hep3B cells were divided into control group and RNF113A overexpression group(RNF113A-OE),HepG2 was divided into control group and RNF113A knockdown group(si-RNF113A),CCK-8 assay was used to detect changes in cell viability,clone formation assay was used to detect changes in cell proliferation abili-ty,Transwell assay was used to detect changes in cell invasion ability,wound healing assay was used to detect changes in cell migration ability,and flow cytometry was used to detect changes in cell apoptosis ability,Western blot experiments were used to detect changes in protein expression of autophagy related genes and AMPK signaling pathway related genes.Results:Compared with the control group,the proliferation,cloning,invasion,and migration abilities of Hep3B cells in the RNF113A-OE group were improved,while apoptosis and autophagy abilities were decreased,and the AMPK signaling pathway was inhibited;In the si-RNF113A group,the proliferation,cloning,in-vasion,and migration abilities of HepG2 cells were significantly reduced,while apoptosis and au-tophagy abilities were increased,and the activation of the AMPK signaling pathway was promoted.Conclusion:RNF113A promotes the proliferation,cloning,invasion,and migration of hepatocel-lular carcinoma cells,and inhibited apoptosis and autophagy by inhibiting the AMPK signaling path-way.
维普
万方数据