Objective:To explore and analyze the mechanism of Fibronectin(FN)targeting JAK/STAT3 signaling pathway to regulate adhesive ileus.Methods:From January 2019 to De-cember 2020,65 patients with adhesive ileus who underwent intestinal resection were selected as the observation group,and 65 patients who underwent inguinal hernia surgery during the same pe-riod were selected as the control group.Intestinal tissue and preoperative blood samples were taken from the patients.qRT-PCR,Western Blot and immunohistochemistry were used to detect FN,STAT3 and P-STAT3 gene and protein expression in intestinal tissue samples,and Enzyme-linked immunosorbent assay(ELISA)was used to detect FN expression in serum samples.Cell line HIEC-6 was cultured in vitro.FN expression was inhibited by lentivirus transfection,and the effects of FN on cell proliferation and differentiation were observed by CCK8 and Transwell migra-tion experiments.At the same time,qRT-PCR and Western Blot were used to detect the effects of FN on the expression of STAT3 and P-STAT3.Results:Compared with the control group,the expression of FN mRNA and protein in intestinal tissue of observation group was significantly higher(P＜0.05),while the expression of STAT3 and P-STAT3 mRNA and protein was significantly lower(P＜0.05),and the content of secreted FN in serum of observation group was also signifi-cantly higher(P＜0.05).In vitro experiments showed that compared with NC siRNA group HIEC-6 cells,the proliferation rate of LV-FN siRNA group HIEC-6 cells was significantly lower at 48 h,72 h,96 h and 120 h(P＜0.05),and the cell migration ability was also significantly lower(P＜0.05).The mRNA and protein expressions of FN were significantly lower(P＜0.05),while the mRNA and pro-tein expressions of STAT3 and P-STAT3 were significantly higher(P＜0.05).Conclusion:Fibro-inin is significantly overexpressed in the intestinal tissues and serum of patients with adhesive il-eus,and can affect cell proliferation and migration through abnormal activation of JAK/STAT3 sig-naling pathway,leading to the pathogenesis of the diseased intestinal segment.
维普
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