Objective:To investigate the application value of indocyanine green fluorescence laparoscopic lateral lymph node dissection(LLND)in the treatment of middle and low rectal can-cer.Methods:Clinical data of 76 patients with middle and low rectal cancer who underwent surgi-cal treatment from February 2018 to June 2020 were retrospectively analyzed,including 42 patients in the TME group who underwent laparoscopic total mesorectal resection and 34 patients in the TME+LLND group who underwent laparoscopic TME combined with indocyanine green fluores-cence LLND.Results:The operation time of TME+LLND group was longer than that of TME group(P<0.001).Compared with TME group,the total number of lymph nodes dissected in TME+LLND group was higher(P<0.05).In the TME+LLND group,7 cases(20.59%)reported lateral lymph node metastasis.After 3 years of follow-up,the local recurrence rate was 2.94%in TME+LLND group and 19.05%in TME group(P<0.05).The distant metastasis rate was 14.71%in the TME+LLND group and 9.52%in the TME group(P>0.05).Conclusion:Indocyanine green fluorescence laparoscopic LLND can reduce the local recurrence rate and does not increase postoperative com-plications,so it is safe,effective and feasible in the treatment of middle and low rectal cancer.