基于脂质组学的整合素αvβ6对结肠癌细胞的促癌机制研究
Effects of integrin αvβ6 on lipidomics metabolism in relation to tumor progression for colon cancer
梁本甲 1付仰彬 1于金涛 1王军 2牛军 3周明良1
作者信息
- 1. 山东第一医科大学附属省立医院 胃肠外科(山东 济南 250021)
- 2. 淄博市张店区人民医院 普外科(山东 淄博 255020)
- 3. 山东大学齐鲁医院 肝胆外科(山东 济南 250012)
- 折叠
摘要
目的:探讨整合素αvβ6对结肠癌细胞的脂质代谢的影响;确定用于诊断结肠癌的潜在代谢生物标志物.方法:利用基于液相色谱-质谱(LC-MS)的脂质组学分析研究αvβ6对结肠癌细胞系SW480代谢变化的影响.偏最小二乘判别分析模型在αvβ6过表达或低表达的SW480中显示出不同的脂质特征.结果:在αvβ6过表达后,SW480细胞中共有252种脂质呈现出显著的差异,其中138种脂质出现上调、114种脂质出现下调.同时发现,甲基磷胆碱(MePC)、磷脂酰胆碱(PC)、磷酰乙醇胺(PE)和甘油三酯(TG)对αvβ6过表达的癌细胞具有良好的诊断潜力.结论:整合素αvβ6可能通过调节脂质代谢失调的途径,使癌细胞更具侵袭性和转移性.这些结果可能为αvβ6对结肠癌细胞作用的分子机制提供潜在的线索.
Abstract
Objective:To investigate the effect of integrin αvβ6 on lipid metabolism in colon cancer cells;to identify potential metabolic biomarkers for the diagnosis of colon cancer.Meth-ods:Liquid chromatography-mass spectrometry(LC-MS)-based lipidomic analysis was utilized to investigate the effects of αvβ6 on the changes in SW480 metabolism in colon cancer cell lines.The partial least squares discriminant analysis model showed different lipid profiles in Integrin αvβ6 over-expressed or low-expressed SW480.Results:After Integrin αvβ6 overexpression,a total of 252 lipids showed significant differences in SW480 cells,of which 138 showed up-regulation and 114 showed down-regulation.Moreover,the Methyl phosphatidylcholine(MePC),phosphatidyl-choline(PC),phosphatidylethanolamine(PE)and triglycerides(TG)were also found to have good diagnostic potential in αvβ6 overexpressing cancer cells.Conclusion:Integrin αvβ6 may promote cancer cell invasivness and metastasis by regulating pathways of dysregulated lipid metabolism.These results may provide potential clues to the molecular mechanisms of Integrin αvβ6 on colon cancer cells.
关键词
结肠肿瘤/整合素αvβ6/脂质组学/脂质代谢Key words
Colon tumor/Integrin αvβ6/Lipidomics/Lipid metabolism引用本文复制引用
基金项目
山东省自然科学基金(ZR2020MH219)
山东省自然科学基金(ZR2023MH088)
出版年
2024
NETL
NSTL
万方数据