血清miR-30c-5p和NLGN1联合检测对腹腔镜结直肠癌根治术患者术后复发转移的预测价值
Predictive value of combined detection of serum miR-30c-5p and NLGN1 for postoperative recurrence and metastasis in patients undergoing laparoscopic radical resection of colorectal cancer
欧洋 1王泽军 2田雾 1杨维敏 1潘俞霖1
作者信息
- 1. 北京积水潭医院贵州医院 普通外科(贵州 贵阳 550000)
- 2. 贵州医科大学附属肿瘤医院 胃肠外科(贵州 贵阳 550000)
- 折叠
摘要
目的:探讨血清微小核糖核酸-30c-5p(miR-30c-5p)和神经角质蛋白1(NLGN1)联合检测对腹腔镜结直肠癌根治术后复发转移的预测价值.方法:选取2021年8月-2022年8月进行腹腔镜结直肠癌根治术的结直肠癌患者112例为病例组,并根据患者术后12个月内是否复发转移分为复发组(n=28)和未复发组(n=84);另选同期进行体检的正常志愿者92例为对照组.qRT-PCR检测血清miR-30c-5p、NLGN1 mRNA水平,多因素Logistic回归分析患者术后复发转移的影响因素,绘制受试者工作特征(ROC)曲线分析血清miR-30c-5p、NLGN1 mRNA对患者术后复发转移的预测价值,Pearson法分析miR-30c-5p与NLGN1 mRNA相关性.结果:病例组miR-30c-5p水平显著低于对照组(P＜0.05),NLGN1 mRNA水平显著高于对照组(P＜0.05);与未复发组相比,复发组miR-30c-5p水平显著降低(P＜0.05),NLGN1 mRNA水平显著升高(P＜0.05),且未复发组与复发组的组织分化程度之间差异有统计学意义(P＜0.05);组织低分化、NLGN1 mRNA水平升高为术后复发转移的危险因素(P＜0.05),miR-30c-5p水平升高为术后复发转移的保护因素(P＜0.05);血清miR-30c-5p、NLGN1 mRNA及联合预测患者术后发生复发转移的AUC为0.823、0.823、0.902,联合预测显著优于miR-30c-5p(Z=2.031,P=0.042)、NLGN1 mRNA(Z=2.239,P=0.025)单独预测;miR-30c-5p 与 NLGN1 mRNA 水平具有负相关性(r=-0.436,P＜0.05).结论:在腹腔镜结直肠癌根治术后复发转移患者中,miR-30c-5p水平降低,NLGN1 mRNA水平升高,对患者术后复发转移具有一定的辅助预测价值.
Abstract
Objective:To investigate the predictive value of combined detection of serum microribonucleic acid-30c-5p(miR-30c-5p)and neuroligin-1(NLGN1)for postoperative recur-rence and metastasis in patients undergoing laparoscopic radical resection of colorectal cancer.Methods:A total of 112 colorectal cancer patients who underwent laparoscopic radical resection from August 2021 to August 2022 were regarded as the diseased group.They were separated into a recurrent group(n=28)and a non-recurrent group(n=84)based on whether the patients had recurrence or metastasis within 12 months after surgery.Additionally,92 normal volunteers who underwent physical examinations were as the control group.QRT-PCR was applied to de-tect serum miR-30c-5p and NLGN1 mRNA levels.Multivariate logistic regression was applied to analyze the affecting factors of postoperative recurrence and metastasis in patients.Receiver op-erating characteristic(ROC)curve was plotted to analyze the predictive value of serum miR-30c-5p and NLGN1 mRNA for postoperative recurrence and metastasis in patients.Pearson method was applied to analyze the correlation between miR-30c-5p and NLGN1.Results:The miR-30c-5p level in the diseased group was greatly lower than that in the control group(P＜0.05),and the NLGN1 mRNA level was significantly higher than that in the control group(P＜0.05).Compared with the non recurrent group,the miR-30c-5p level in the recurrent group was significantly re-duced(P＜0.05),while the NLGN1 mRNA level was significantly increased(P＜0.05).There was a statistically significant difference in tissue differentiation between the non-recurrent group and the recurrent group(P＜0.05).Low differentiation of tumor tissues and elevated level of NLGN1 mRNA were risk factors for postoperative recurrence and metastasis(P＜0.05).Elevated level of miR-30c-5p was a protective factor of postoperative recurrence and metastasis(P＜0.05).The AUC of serum miR-30c-5p,NLGN1 mRNA,and their combined prediction for postoperative re-currence and metasta sis in patients was 0.823,0.823,and 0.902,which was greatly better than the individual prediction of miR-30c-5p(Z=2.031,P=0.042)and NLGN1 mRNA(Z=2.239,P=0.025).There was a negative correlation between miR-30c-5p and NLGN1 mRNA levels(r=-0.436,P＜0.05).Conclusion:The dicrease of miR-30c-5p level and increase of NLGN1 mRNA level in laparoscopic colorectal cancer patient has auxiliary predictive value for postoperative recur-rence and metastasis.
关键词
微小核糖核酸-30c-5p/神经角质蛋白1/结直肠肿瘤/联合检测/复发/转移Key words
Microribonucleic acid-30c-5p/Neuroligin-1/Colorectal tumour/Combine test/Recurrence/Metastasis引用本文复制引用
基金项目
2021年贵州省卫生健康委科学技术基金项目(GZWKJ2021-224)
出版年
2024
NETL
NSTL
万方数据