Objective This study aims to investigate the efficiency of puerarin targeting the Akt signaling pathway in intervening with Human Immunodeficiency Virus(HIV)infection in T cells.Methods Jurkat T cells were cultured,and the impact of puerarin intervention on Jurkat T cell activity was assessed using WST-1 reagent.Jurkat T cells were divided into a blank control group,puerarin group,LY294002 group(PI3K inhibitor),and a combined puerarin-LY294002 group.After 24 hours of drug intervention,real-time quantitative polymerase chain reaction(qRT-PCR)was employed to measure the relative expression of PI3K and Akt mRNA in each group,and Western blotting was used to detect the relative expression of p-PI3K/PI3K and p-Akt/Akt proteins.HIV pseudovirus was prepared to infect cells in each group,and the efficiency of pseudovirus infection in Jurkat T cells was assessed using a luciferase system.Results WST-1 assay results revealed a dose-dependent reduction in Jurkat T cell viability with increasing concentrations of puerarin(P<0.05).qRT-PCR and Western blotting results indicated that puerarin decreased the relative expression of PI3K and downstream Akt mRNA,as well as p-PI3K/PI3K and p-Akt/Akt proteins in Jurkat T cells(P<0.05).Results from the HIV pseudovirus infection experiment demonstrated that puerarin reduced the efficiency of HIV pseudovirus infection in Jurkat T cells,with the combined treatment showing superior inhibitory effects compared to the individual treatment(P<0.05).Conclusion Puerarin may reduce the activation of Akt downstream of PI3K,thereby decreasing the opportunity for HIV pseudovirus spread and entry into Jurkat T cells,ultimately lowering the probability of infection.
关键词
艾滋病/穿心莲内酯/人类免疫缺陷假病毒/PI3K/Akt
Key words
acquired immunedeficiency syndrome/andrographolide/human immunodeficiency pseudovirus/phosphoinositide 3-kinase/protein kinase b
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