Objective To observe the therapeutic effects of Gubitongxiao granules on hormone-induced femoral head necrosis(SONFH)in mice and explore its mechanism of action based on the receptor activator of nuclear factor-κB ligand/receptor activator of nuclear factor-κB/osteoprotegerin(RANKL/RANK/OPG)signaling pathway.Methods A SONFH animal model was established by intraperitoneal injection of lipopolysaccharide and intramuscular injection of methylprednisolone acetate into the gluteus of 60 mice.After confirmation by magnetic resonance imaging,the mice were divided into a model group(MC),a Gubitongxiao granules group(GBTX),and a Tongluo Shenggu Gelatin Capsules group(PC),with 20 mice in each group.An additional 12 normal mice were used as a blank control group(NC).Corresponding drugs were administered orally to each group for 12 weeks.Subsequently,under anesthesia,samples were collected,and the general behaviors of the mice were observed.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of bone alkaline phosphatase(BALP)and type I procollagen N-terminal propeptide(PINP)in the mice.Western blotting and real-time quantitative polymerase chain reaction(qRT-qPCR)were used to measure the protein and gene expression levels of RANK,RANKL,OPG,phospholipase Cγ2(PLCγ2),cathepsin K(CTSK),and tartrate-resistant acid phosphatase(TRAP)in each group.Results Magnetic resonance imaging showed a high signal state in the left hip of the mice after successful model replication.Compared with the NC group,the MC group showed decreased levels of BALP,PINP,OPG protein,and gene expression(P<0.05),and increased levels of RANK,RANKL,PLCγ2,CTSK,and TRAP protein and gene expression(P<0.05).Compared with the MC group,the GBTX and PC groups showed increased levels of BALP,PINP,OPG protein,and gene expression(P<0.05),and decreased levels of RANK,RANKL,PLCγ 2,CTSK,and TRAP protein and gene expression(P<0.05).Compared with the GBTX group,the PC group showed no significant differences in OPG,RANK,RANKL,CTSK,and TRAP protein and gene expression(P>0.05),while the gene expression of PLCγ2 in the PC group was lower than that in the GBTX group(P<0.05).Conclusion Gubitongxiao granules may improve femoral head necrosis by upregulating OPG expression,inhibiting RANK,RANKL,PLCγ2,CTSK,and TRAP expression,thereby ameliorating the condition.
steroid-induced femoral head necrosisGubitongxiao granulesRANKL/RANK/OPG signaling axis
维普
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