Objective To explore the role and underlying mechanism of Bushen Jiangzhuo Recipe in diabetic nephropathy(DN).Methods A rat model of DN was established and treated with Bushen Jiangzhuo Recipe at a dose of 11.04 g/(kg·d).Based on different treatment,the rats were randomly divided into the control group,DN group,and DN + Bushen Jiangzhuo Recipe group,with 6 in each group.The levels of urinary albumin,blood urea nitrogen(BUN)and serum creatinine(Scr)were measured by biochemical analyzer.The pathological damage of renal tissues was detected by H&E staining,and the renal fibrosis was detected by Masson staining.The mRNA expression level of SIRT1 was detected by qRT-PCR,and the expression of proteins associated with the MAPK/NF-κB signaling pathway was detected by Western blotting.Results The levels of BUN,Scr and urinary albumin in the DN group were higher than those in the control group(P<0.05),while those in the DN + Bushen Jiangzhuo Recipe group were lower compared with the DN group(P<0.05).The relative mRNA expression of SIRT1 in the DN group was lower than that in the control group(P<0.05),whereas that in the DN + Bushen Jiangzhuo Recipe group was higher compared with the DN group(P<0.05).The glomeruli and tubules were morphologically intact in the control group,while thickening of the tubular basement membrane and the glomerular hypertrophy were observed in the DN group,indicating severe damage to the renal function of rats in the DN group.In comparison to the DN group,the thickening of the tubular basement membrane and the glomerular hypertrophy were mitigated in the DN + Bushen Jiangzhuo Recipe group.Compared with the control group,increased collagen deposition in glomerulus and the tubular lumen were observed in the DN group.There was a reduction in the collagen deposition in glomerulus and the tubular lumen in the DN + Bushen Jiangzhuo Recipe group compared with the DN group.The protein expression of SIRT1 in the DN group was lower than that in the control group(P<0.05),while the levels of p-p38/p38 and p-NF-κB p65/NF-κB p65 in the DN group were higher than those in the control group(P<0.05).The protein expression of SIRT1 in the DN + Bushen Jiangzhuo Recipe group was higher than that in the DN group(P<0.05),while the levels of p-p38/p38 and p-NF-κB p65/NF-κB p65 in the DN + Bushen Jiangzhuo Recipe group were lower than those in the DN group(P<0.05).Conclusion Bushen Jiangzhuo Recipe ameliorates the renal tissue damage in DN via upregulating the expression of SIRT1 and inhibiting the MAPK/NF-κB signaling pathway.
国家科技期刊平台
NSTL
维普
万方数据
