Objective To explore the function and potential mechanism of LncRNA ZEB2-AS1 combined with its parental gene ZEB2 in ovarian cancer SKOV3 cells.Methods In situ hybridization and immunohistochemistry were used to detect the expression of ZEB2-AS1 and ZEB2 in ovarian cancer tissues.SKOV3 cell models with ZEB2-AS1 overexpression and silencing were established.Cell proliferation was measured using the CCK-8 assay.Flow cytometry was used to detect cell apoptosis and cycle.Epithelial-mesenchymal transition(EMT)-related protein expression was assessed through immunofluorescence and Western blotting.Dual-luciferase gene reporter assays were performed to confirm the binding relationship between ZEB2-AS1,ZEB2,and microRNA-145(miR-145).Results ZEB2-AS1 and ZEB2 were expressed in the cytoplasm and nucleus of ovarian cancer tissues.Compared with SKOV3 cells,ZEB2-AS1 overexpressing SKOV3 cells showed increased proliferation(P＜0.05),reduced apoptosis(P＜0.05),enhanced invasion and migration(P＜0.05),increased S-phase cells(P＜0.05),and decreased G,phase cells(P＜0.05).EMT-related protein expression showed a decrease in E-cadherin(P＜0.05)and an increase in N-cadherin and Vimentin(P＜0.05).Conversely,ZEB2-AS1 silenced SKOV3 cells exhibited opposite trends.Dual-luciferase reporter assay confirmed the binding relationship between ZEB2-AS1 and miR-145,as well as between ZEB2-AS1 and ZEB2 mRNA.Conclusions LncRNA ZEB2-AS1 combined with its parental gene ZEB2 promotes EMT in ovarian cancer SKOV3 cells.
国家科技期刊平台
NSTL
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