Objective To explore the changes in ILC2s and their associated moleculars in the peripheral blood of multiple myeloma(MM)patients.Methods This study included sixty MM patients and forty healthy controls.The proportion of ILC2s cells and the expression of KLRG1 on these cells were analyzed using flow cytometry,while the mRNA levels of GATA-3,ST2,IL-5,IL-13,and IL-7RB were quantified through PCR.Results In newly diagnosed MM patients,there was an increase in the proportion of ILC2s,KLRG1 expression on ILC2s,and the mRNA levels of GATA-3,ST2,IL-5,and IL-13 in PBMC(P＜0.05).Additionally,the proportion of ILC2s cells showed a positive correlation with these associated molecules(r=0.831,0.748,0.737,0.702,and 0.699,P＜0.05).Post-treatment,the remission group exhibited lower levels of ILC2s cells,KLRG1 expression on ILC2s,and mRNA levels of GATA-3,ST2,IL-5,and IL-13 compared to the non-remission group(P＜0.05).Furthermore,these parameters decreased in the remission group post-treatment compared to pre-treatment(P＜0.05).Conversely,in the non-remission group,the proportion of ILC2s cells and associated molecular levels significantly increased post-treatment(P＜0.05).ROC curves demonstrated that pre-treatment levels of KLRG1 expression on ILC2s cells and IL-13 mRNA had significant predictive value for treatment efficacy.Conclusion ILC2s cells contribute to immune dysregulation in MM,promoting tumor growth.These cellular immune disorders may result from alterations in cell-associated effector molecules.
国家科技期刊平台
NSTL
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