Objective To explore the protective effect and mechanism of nicotinamide riboside(NR)on SH-SY5Y cells damage induced by MPP+.Methods SH-SY5Y cells in the logarithmic growth stage were used in the experiment.Cells were treated with 3 mmol/L MPP+to establish Parkinson disease(PD)model.The cells were divided into control group(cells were cultured normally),MPP+group(cells treated with MPP+),NR group(cells treated with MPP+and 0.5 mmol/L NR).After culturing,cell survival rate,apoptosis rate,oxidative stress level[reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD)],inflammation level[interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)]and nicotinamide adenine dinucleotide(NAD+)levels were detected.Results Compared with the control group,the cell apoptosis,ROS,MDA content,TNF-α,and IL-6 were increased,while the cell survival rate,SOD activity and NAD+levels were decreased(P<0.05)in the MPP+group.Compared with the MPP+group,the ROS,MDA content,TNF-α,and IL-6 levels were significantly decreased,while the cell survival rate,SOD activity and NAD+levels were significantly increased(P<0.05)in the NR group.Conclusion NR attenuates MPP+-induced injury in neural cells,which may be involved in oxidative stress and inflammation through NAD+supplementation.
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