Objective To analyze the role of transcutaneous electrical nerve stimulation(TENS)in alleviating fatigue in sleep-deprived mice and to explore the potential neurohumoral mechanisms involved.Methods A total of 18 SPF-grade Kunming mice were selected and numbered according to their body weight.They were randomly divided into three groups:the electrical stimulation+sleep deprivation group,the sleep deprivation group,and the normal group,with six mice in each group.Mice in the electrical stimulation+sleep deprivation group underwent 20 hours of sleep deprivation daily,combined with TENS intervention.The sleep deprivation group received only the 20 hours of sleep deprivation,while no intervention was performed on the normal group.After 7 days of intervention,all three groups underwent tail suspension and open field tests to collect relevant behavioral data.Serum levels of corticotropin-releasing factor and corticosterone(CORT)in each group were measured using competitive inhibition Enzyme-linked Immunosorbent Assay.Results The results from the tail suspension test indicated that the percentage of immobility time in the electrical stimulation+sleep deprivation group was lower than that in the sleep deprivation group(P<0.05),while the percentage of struggling time in the electrical stimulation+sleep deprivation group was higher(P<0.05).In the open field test,the time spent in the central area by the electrical stimulation+sleep deprivation group was longer compared to the sleep deprivation group(P<0.05).Serum hormone level analysis showed that serum CORT levels in the sleep deprivation group were higher than those in the normal group(P<0.05).Conclusion TENS can ameliorate anxiety,depression,and other behavioral changes induced by sleep deprivation in mice,which may be related to abnormal hormone levels in the hypothalamic-pituitary-adrenal(HPA)axis.TENS holds potential value in relieving mental fatigue caused by sleep deprivation.
关键词
睡眠剥夺/精神疲劳/经皮神经电刺激疗法/悬尾实验/旷场实验
Key words
sleep deprivation/mental fatigue/transcutaneous electrical nerve stimulation/mouse tail suspension test/open field test
维普
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