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两种艾滋病抗体检测方法在艾滋病诊断中的作用

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目的 分析酶联免疫法和胶体金法在艾滋病抗体检测中的使用效果。方法 选取 45 例艾滋病抗体检测阳性患者及 45 例健康体检者为研究对象,所有受检者均接受酶联免疫法和胶体金法艾滋病抗体检测。分析两种检测方法的艾滋病检出情况,比较两种检测方法的诊断敏感性、特异性。结果 酶联免疫法检出艾滋病阳性43例,阳性检出率为47。78%(43/90);胶体金法检出艾滋病阳性44例,阳性检出率为 48。89%(44/90)。酶联免疫法的诊断敏感性为 95。56%(43/45),特异性为 100。00%(45/45);胶体金法的诊断敏感性为 97。78%(44/45),特异性为 100。00%(45/45)。酶联免疫法和胶体金法的诊断敏感性、特异性比较,差异无统计学意义(P>0。05)。结论 艾滋病对人体危害较大,会破坏患者自身免疫屏障,降低患者自身免疫力,损伤患者内脏器官。患者应尽早筛查并采取治疗措施,避免病情继续发展。目前采取酶联免疫法和胶体金法均能够获得较好的检测结果,胶体金法常用于窗口期检测,而酶联免疫法主要用于初筛,在实际检测过程中可以结合实际情况选择适合的检测方式。
Two AIDS antibody test methods in AIDS diagnosis
Objective To analyze the effect of enzyme-linked immunoassay and colloidal gold assay in AIDS diagnosis.Methods 45 HIV-positive patients and 45 healthy subjects were selected as the study subjects,and all received HIV antibody detection by enzyme-linked immunoassay and colloidal gold assay.The AIDS detection of the two methods was analyzed,and the diagnostic sensitivity and specificity of the two methods were compared.Results Enzyme-linked immunoassay detected 43 HIV-positive cases,and the positive detection rate was 47.78%(43/90).Colloidal gold assay detected 44 HIV-positive cases,and the positive detection rate was 48.89%(44/90).The diagnostic sensitivity and specificity of enzyme-linked immunoassay were 95.56%(43/45)and 100.00%(45/45).The diagnostic sensitivity and specificity of colloidal gold assay were 97.78%(44/45)and 100.00%(45/45).There was no statistically significant difference in diagnostic sensitivity and specificity between enzyme-linked immunoassay and colloidal gold assay(P>0.05).Conclusion AIDS is harmful to human body,which can destroy the autoimmune barrier,reduce the immunity of patients,and damage the internal organs of patients.Patients should be screened early and treated to prevent further progression of the disease.At present,both enzyme-linked immunoassay and colloidal gold assay can obtain better detection results.Colloidal gold assay is often used for window period detection,while enzyme-linked immunoassay is mainly used for preliminary screening.In the actual detection process,appropriate detection methods can be selected according to the actual situation.

AIDSDiagnosisAntibody detectionEnzyme-linked immunoassayColloidal gold assay

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110021 沈阳市铁西区疾病预防控制中心

艾滋病 诊断 抗体检测 酶联免疫法 胶体金法

2024

中国现代药物应用
中国水利电力医学科学技术学会

中国现代药物应用

影响因子:0.862
ISSN:1673-9523
年,卷(期):2024.18(1)
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