Objective To analyze the effect of enzyme-linked immunoassay and colloidal gold assay in AIDS diagnosis.Methods 45 HIV-positive patients and 45 healthy subjects were selected as the study subjects,and all received HIV antibody detection by enzyme-linked immunoassay and colloidal gold assay.The AIDS detection of the two methods was analyzed,and the diagnostic sensitivity and specificity of the two methods were compared.Results Enzyme-linked immunoassay detected 43 HIV-positive cases,and the positive detection rate was 47.78%(43/90).Colloidal gold assay detected 44 HIV-positive cases,and the positive detection rate was 48.89%(44/90).The diagnostic sensitivity and specificity of enzyme-linked immunoassay were 95.56%(43/45)and 100.00%(45/45).The diagnostic sensitivity and specificity of colloidal gold assay were 97.78%(44/45)and 100.00%(45/45).There was no statistically significant difference in diagnostic sensitivity and specificity between enzyme-linked immunoassay and colloidal gold assay(P>0.05).Conclusion AIDS is harmful to human body,which can destroy the autoimmune barrier,reduce the immunity of patients,and damage the internal organs of patients.Patients should be screened early and treated to prevent further progression of the disease.At present,both enzyme-linked immunoassay and colloidal gold assay can obtain better detection results.Colloidal gold assay is often used for window period detection,while enzyme-linked immunoassay is mainly used for preliminary screening.In the actual detection process,appropriate detection methods can be selected according to the actual situation.