Intestinal flora diversity of malnourished children based on high-throughput sequencing
Objective To compare and analyze the diversity and actual composition characteristics of proposed flora species between malnourished children and normal children by using high-throughput sequencing mode and method.Methods 20 malnourished children were selected as group A,and 20 children with normal health were selected as the control group.Stool samples were collected for analysis and discrimination,and ribosomal DNA(RDNA)in bacteria was extracted.Through two groups of polymerase chain reaction(PCR)models and methods,high-throughput sequencing design was achieved by increasing V4-V5 regions.In addition,the bioinformatics analysis and research were carried out,the corresponding dilution curve was constructed,the MOTHUR software was used to realize the diversified calculation and analysis of APLHA,the HEAT MAP analysis and research were realized through R language,and the canonical correspondence analysis(CCA)was realized.After that,the data were integrated and summarized in the two lists of sample gate and genus,respectively,for statistical analysis.Results There was no statistically significant difference between the two groups in the basic information of age,gender,mode of delivery(P>0.05).There was no statistically significant difference in biodiversity between the two groups(P>0.05).A total of 154 microbial species were found in the two groups of samples.The operational taxonomic unit(OTU)of intestinal flora of group A was 320,the OTU of group B colorectal methanogens was 348,and their common OTU was 290.A total of 14 species of intestinal flora were detected in the two groups of children.In order to better display the test results,this study relied on the advantages of bar charts to compare and study each sample at the taxonomic level.OTU of Firmicutes and ungraded bacteria(lactic acid bacteria)in group A was less than that in group B,and OTU of Bacteroides(Bacteroides thetaiotaomicron and Rothia)was more than that in group B.The difference was statistically significant(P<0.05).In addition to the flora contained in group A,group B also had 19 unique species such as Anaerovibrio and Anaerobiospirillum,which far exceeded the species and abundance of flora in group A.The abundance of 5 bacterial communities in the two groups was the same,including Bacteroides thetaiotaomicron and Arcanobacterium,but the difference was not statistically significant(P>0.05).The abundance of other bacterial communities showed statistical significance(P<0.05).Conclusion The number of Firmicutes in the intestinal flora of malnourished children is low,and the content of Bacteroides thetaiotaomicron and Rothia is high.Therefore,in order to ensure the stability and balance of the flora,it is necessary to adjust the colony by taking advantage of the actual characteristics of different bacteria genera to ensure that children have good absorption and physical development.
High-throughput sequencingMalnutritionChildren's intestinesDiversity of flora
NETL
NSTL
万方数据
维普
