Effect of Zhenwu Decoction on renal function,proteinuria and hemorheology in diabetic nephropathy rats
Objective To analyze the effect of Zhenwu Decoction on renal function,proteinuria and hemorheology in diabetic nephropathy rats and its potential mechanism.Methods 24 male SD rats were divided into normal group(8 rats)and model group(16 rats).The model group was constructed as a diabetic nephropathy model by high-sugar and high-fat feeding and intraperitoneal injection of streptozotocin(STZ)(65 mg/kg),and the rats in the model group after the successful construction were subdivided into the model-control group(8 rats,10 ml of normal saline was given by gavage)and the Zhenwu Decoction group[8 rats,5.0 g/(kg·d),10 ml/kg].At the 27th week of the experiment,blood and 24 h urine samples of the three groups of rats were collected for biochemical examination,hemorheological indexes were determined,and the levels of nephrin and podocin in renal tissues were analyzed by reverse transcription polymerase chain reaction(RT-PCR).Results(i)At the 27th week of the experiment,the levels of fasting blood glucose(FBG),24-h urinary protein quantity,blood urea nitrogen(BUN)and serum creatinine(Cr)in the normal group were(6.42±0.83)mmol/L,(5.12±0.76)mg/24 h,(8.41±0.42)mmol/L and(104.48±18.72)μmol/L;those in the control group were(21.24±2.22)mmol/L,(27.23±4.10)mg/24 h,(26.27±1.74)mmol/L and(311.91±19.94)μmol/L;those in Zhenwu Decoction group were(20.22±2.19)mmol/L,(10.12±1.87)mg/24 h,(12.94±2.02)mmol/L and(194.95±17.42)μmol/L.There were significant differences in FBG,24 h urinary protein quantity,BUN and Cr among the three groups(P＜0.05).Compared with normal group,FBG,24 h urinary protein quantity,BUN and Cr in model-control group and Zhenwu Decotion group were significantly increased(P＜0.05).Compared with model-control group,the 24 h urinary protein quantity,BUN and Cr in Zhenwu Decoction group were significantly decreased(P＜0.05).(ii)At the 27th week of the experiment,the hematocrit,erythrocyte count,erythrocyte aggregation index and erythrocyte deformation index in the normal group were(0.48±0.05)L/L,(5.33±0.31)×1012/L,(8.10±0.75)and(0.95±0.08);those in the control group were(0.57±0.03)L/L,(5.89±0.34)×1012/L,(11.20±1.96)and(0.84±0.05);those in the Zhenwu Decoction group were(0.52±0.01)L/L,(5.60±0.15)×1012/L,(9.94±1.45)and(0.93±0.04).There were significant differences in hematocrit,erythrocyte count,erythrocyte aggregation index and erythrocyte deformation index among the three groups(P＜0.05).Compared with normal group,the hematocrit,erythrocyte count and erythrocyte aggregation index of model-control group and Zhenwu Decoction group were significantly increased,while the erythrocyte deformation index of model-control group was significantly decreased(P＜0.05).Compared with the model-control group,the hematocrit,erythrocyte count and erythrocyte aggregation index of Zhenwu Decoction group were significantly decreased,and the erythrocyte deformation index was significantly increased(P＜0.05).(iii)At the 27th week of the experiment,the expression levels of nephrin and podocin in the normal group were(1.80±0.09)and(1.94±0.08),those in the control group were(1.00±0.07)and(0.99±0.05),and those in the Zhenwu Decoction group were(1.54±0.06)and(1.61±0.07).The expression levels of nephrin and podocin in three groups were significantly different(P＜0.05).Compared with normal group,the nephrin and podocin in model-control group and Zhenwu Decoction group were significantly decreased(P＜0.05).Compared with model-control group,nephrin and podocin in Zhenwu Decoction group were significantly increased(P＜0.05).Conclusion Zhenwu Decoction can effectively reduce kidney injury and improve renal function in rats with diabetic nephropathy by improving metabolic disorders,increasing nephrin and podocin gene expression.Zhenwu Decoction may be an effective way to treat diabetic nephropathy.
万方数据
维普
